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| Study on the inhibition of SiHa cell activity by regulating Foxp3 and affecting the β-catenin pathway with the extract of Qingdushuan |
| Hits 1156 Download times 743 Received:November 09, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.04.22 |
| Key Words
Foxp3 overexpressed plasmid;Qingdushuan;cervical cancer;SiHa cell;β-catenin pathway |
| Author Name | Affiliation | E-mail | | GENG Weihua | Beijing University of Chinese Medicine, Beijing 100029, China | | | LOU Jiaoying | Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China | doctorlou@126.com | | ZHANG Jing | Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Ying | Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Mei | Beijing University of Chinese Medicine, Beijing 100029, China | |
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| Abstract
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| [Objective] To investigate the effect of Qingdushuan on the activity of SiHa cell in cervical cancer,and to explore its possible mechanism based on forkhead/winged helix transcription factor (Foxp3).[Methods] The method of Liposome-mediated transfection was used to establish Foxp3 overexpressed human papilloma virus (HPV) -positive SiHa cell lines,and pcDNA3.1 empty vector transfected SiHa cell lines were as the negative control. Western Blot method was used to detect the levels of Foxp3 proteins and their downstream signaling molecules in the Foxp3 overexpression plasmid group and blank plasmid group SiHa cells treated with empty white blood and 15% drug-containing serum respectively. CCK8 method was used to detect SiHa cell activity in the four groups.[Results] Foxp3 overexpression vector transfection treatment can significantly increase the protein content of Foxp3 protein,Foxp3 downstream signaling molecules β-catenin,proto-oncogene c-Myc,and cyclin D1 in SiHa cells. Increased SiHa cell activity (P<0.01),while inhibiting the apoptosis gene Caspase-3 shearing (P<0.05). Qingdu Suppository containing serum can significantly inhibit Foxp3 protein (P<0.01) and Foxp3 downstream signaling molecule protein (P<0.05) and inhibited SiHa cell activity (P<0.01),promoted Caspase-3 splicing (P<0.01);Foxp3 overexpression plasmid can inhibit Foxp3 down-regulation and decreased activity in SiHa cells induced by drug-containing serum. It can inhibit the down-regulation of β-catenin,c-Myc and cyclinD1 induced by medicated serum and increase of Caspase-3 shear.[Conclusion] Foxp3 can increase the activity of SiHa cells by activating the β-catenin pathway,which plays the role of proto-oncogene in SiHa cells. However,serum of Qingdushuan can inhibit the activity of SiHa cells by regulating Foxp3 to affect the β-catenin pathway. |
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