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| Mechanism of Sanye Tangzhiqing Tablet in treating type 2 diabetes mellitus based on network pharmacology |
| Hits 2049 Download times 753 Received:December 02, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.04.23 |
| Key Words
Sanye Tangzhiqing Tablet;T2DM;network pharmacology;KEGG pathway enrichment;potential target |
| Author Name | Affiliation | E-mail | | YAN Yiqi | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HUANG Ming | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Yajun | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Lin | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HU Zhen | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | HAN Guoying | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Han | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | zhanghan0023@126.com |
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| Abstract
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| [Objective] To investigate the mechanism of Sanye Tangzhiqing Tablets in the treatment of type 2 diabetes mellitus (T2DM).[Methods] The SYSDT operation of TCMSP (Pharmacology database and analysis platform of traditional Chinese medicine system) was used to determine the effective active ingredients and related targets information of Sanye Tangzhiqing Tablets. Perl language and UniProt database were used to get the gene target reduction of Sanye Tangzhiqing Tablets. The HPO (human phenotype ontology) database and GENECARD database were used to retrieve the target genes related to type 2 diabetes. The PPI (protein interaction network) was drawn by using string database analysis and Cytoscape 3.7.1 software. The PPI was converted into gene number by perl language. The pathway enrichment analysis and go enrichment analysis of KEGG (Kyoto Encyclopedia of gene and genome) were carried out by using bioconducor package of R language. It laid the foundation to explore the multiple pharmacological mechanism of Sanye Tangzhiqing Tablets by the next experimental verification.[Results] Through the network parameter analysis,80 active components were identified,corresponding to 82 biological targets. The 540 T2DM targets were obtained from GENECARD and HPO databases,among which targets with score greater than 30 were selected from GENECARD database. The overlapping targets were extracted and compared with 80 active components to construct "active component target" network. The interaction between the active components of triglyceride and T2DM targets was revealed,and the PPI of overlapping targets was analyzed to identify the key targets of drug affecting diseases. In addition,the enrichment analysis of KEGG pathway and go function was carried out to guide the future experiments.[Conclusion] Sanye Tangzhiqing Tablets has the characteristics of multi-component,multi-target and multi-channel in the treatment of T2DM,which provides a new idea and scientific basis for further research on the molecular mechanism of the anti-diabetic effect of Sanye Tangzhiqing Tablets. |
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