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| Quality analysis of Bletilae Rhizoma slices from different producing areas based on the militarine content determination and establishment of fingerprint by UPLC |
| Hits 1557 Download times 834 Received:December 28, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.05.20 |
| Key Words
Bletilae Rhizoma slice;UPLC;content determination;fingerprint;quality analysis |
| Author Name | Affiliation | E-mail | | LIU Na | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | YUAN Jinfeng | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | PENG Shitao | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | CHAI Chongchong | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | WANG Jingyue | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | ZHANG Kai | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | LI Xinxin | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | | | LI Fei | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China | lf668@sina.com |
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| Abstract
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| [Objcetive] Ultra High Performance Liquid Chromatography(UPLC) was used to determine the content of militarine and establish UPLC fingerprints of Bletilae Rhizoma slices from different producing areas,so as to provide reference for the quality control of Bletilae Rhizoma slices.[Methods] An ACQUITY UPLC® BEH C18 column (2.1 mm×150 mm,1.7 μm) was used to perform gradient elution with acetonitrile-pure water as the mobile phase. The flow rate was 0.3 mL/min,the detection wavelength was 270 nm,and the column temperature was 46℃. The fingerprints of Bletilae Rhizoma slices were established and similarity evaluation were performed. Based on the content determination and fingerprinting,cluster analysis and principal component analysis method were used to evaluate the quality of Bletilae Rhizoma slices from different producing areas.[Results] The content of militarine in 24 batches of Bletilae Rhizoma slices was ranging from 18.98~44.87 mg/g,and the fingerprint was established and 12 common peaks were identified. The similarity of these fingerprint was between 0.812~0.976. Notably,the similarity of 22 batches of Bletilae Rhizoma slices was greater than 0.9,which indicates that there is little difference in the overall quality of commercially available Bletilae Rhizoma slices. Moreover,samples with low content of militarine and 4 critical components in the fingerprint were grouped into one class by cluster analysis and principal component analysis,which had little relationship with the level of similarity.[Conclusion] The method of militarine content determination and establishment of fingerprint by UPLC used to evaluate the quality of Bletilae Rhizoma slice is scientific and feasible. |
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