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| Effect of Huanglian Dihuang Decoction on the expression of CTGF and FN1 in myocardium of rat with diabetic cardiomyopathy |
| Hits 1274 Download times 793 Received:January 15, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.06.23 |
| Key Words
Huanglian Dihuang Decoction;streptozotocin;diabetic cardiomyopathy;rat |
| Author Name | Affiliation | | YIN CHAO | Third Department of Endocrinology, Baoding First Central Hospital, Baoding 071000, China | | WANG Pengyu | Third Department of Endocrinology, Baoding First Central Hospital, Baoding 071000, China | | HU Yanyun | Third Department of Endocrinology, Baoding First Central Hospital, Baoding 071000, China | | MA Chaopeng | Third Department of Endocrinology, Baoding First Central Hospital, Baoding 071000, China | | WANG Xiaoyan | Third Department of Endocrinology, Baoding First Central Hospital, Baoding 071000, China |
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| Abstract
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| [Objective] To study the preventive and therapeutic effects of Huanglian Dihuang Decoction on diabetic cardiomyopathy.[Methods] Eighty SPF SD rats were divided into blank control group,model control group,Huanglian Dihuang Decoction low-dose group (2.7 g/kg crude drug),Huanglian Dihuang Decoction medium-dose group (5.4 g/kg crude drug),and Huanglian Dihuang Decoction high-dose group (10.8 g/kg crude drug),metformin combined with benazepril control group (90 mg/kg,0.9 mg/kg). Except the blank control group,the other rats were first fed with high-fat and high-sugar diet for 6 weeks,and then the rats in each group were injected with streptozotocin (STZ) at a concentration of 44 mg/kg intraperitoneally. After 3 days,fasting blood glucose was measured,and a type 2 diabetes model with a blood glucose greater than 11.1 mmol/L was selected. Rats whose blood glucose met the standard continued to be fed with high-fat diet for 12 weeks. Fasting blood glucose was measured 1 hour after the last administration,and then rats in each group were anesthetized with 5% chloral hydrate (6 mL/kg),blood was taken from the abdominal aorta,and serum LDL-C,TCH,SOD,and MDA were measured. Hearts were fixed with formalin and used for HE staining and immunohistochemical detection of CTGF and FN1 expression in cardiomyocytes.[Results] The results of this study indicate that Huanglian Dihuang Decoction can reduce fasting blood glucose,LDL-C and TC content and the expression of connective tissue growth factor of cardiomyocytes in diabetic cardiomyopathy model rats,and increase its antioxidant capacity and fibronectin expression.[Conclusion] Huanglian Dihuang Decoction has protective effect on cardiomyocytes in diabetic cardiomyopathy model rats. |
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