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| Study of HPLC fingerprint of Descurainiae semen before and after stir-fry without additional ingredients |
| Hits 1089 Download times 811 Received:March 08, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.06.24 |
| Key Words
Descurainiae semen;stir-fry without additional ingredients;HPLC;fingerprint |
| Author Name | Affiliation | E-mail | | WANG Liang | Zhengzhou Agricultural Product Quality Inspection and Circulation Center, Zhengzhou 450001, China | | | LI Hongwei | School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China | lhw20091068@sina.com |
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| Abstract
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| [Objective] To establish and compare the fingerprint of the crude and the processed seeds from Deseurainia sophia (L.) Webbex Prantl.[Methods] HPLC method was adopted on NUCLEODUR C18 column (4.6 mm×250 mm,5 μm),with acetonitrile -0.1 mol/L sodium dihydrogen phosphate solution as the mobile phase for gradient elution. The flow rate was 1.0 mL/min,and the column temperature was maintained at 30℃. The detection wavelength was 254 nm,and the injection volume was 5 μL.[Results] There were 11 common peaks in HPLC fingerprint of crude and processed Descurainiae semen. There are differences of the similarity between various samples and the automatically generated reference fingerprints of crude Descurainiae semen. The similarity between various samples and the automatically generated reference fingerprints of processed Descurainiae semen reached above 0.89. There were differences in fingerprint of Descurainiae semen before and after processing. The common peak areas of No. 23,No. 26,No. 30,No. 35 peak were increased significantly in processed Descurainiae semen,but the common peak areas of No. 9,No.13,No.14,No.41 peak were decreased significantly. There were certain differences of Descurainiae semen between Anguo,Zhouzhi,Minxian,Changzhi and the remaining 8 producing areas,whether their crude or processed Descurainiae semen by cluster analysis. The similarity between 12 batch samples were similarityimproved by stir-fry without additional ingredients.[Conclusions] The established method can provide experimental basis for processing technology and quality standards of Descurainiae semen fried without additional ingredients. |
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