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| Preparation and quality evaluation of luteolin nanosuspension |
| Hits 1170 Download times 763 Received:March 22, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.06.25 |
| Key Words
luteolin;nanosuspension;wet media milling method;single factor experiment design;Box-Behnken experimental design;in vitro dissolution |
| Author Name | Affiliation | E-mail | | XIE Hui | Department of Pharmacy, Hubei 672 Traditional Chinese and Western Medicine Orthopedic Hospital, Wuhan 430070, China | | | HE Jiyan | Department of Pharmacy, Hubei 672 Traditional Chinese and Western Medicine Orthopedic Hospital, Wuhan 430070, China | 290885101@qq.com |
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| Abstract
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| [Objective] To prepare luteolin nanosuspension by wet media milling method and evaluate its quality.[Methods] The single-factor experimental design method was used to optimize the process parameters and determine the types of stabilizers and surfactants in the formulation. The particle size distribution and Zeta potential of luteolin nanosuspension were used as evaluation indicators,the luteolin concentration,the stabilizer concentration and the surfactant concentration were taken as the investigation factors,the formulation of luteolin nanosuspension was optimized by Box-Behnken experiment design method. The microscopic morphology of the luteolin nanosuspension was observed by scanning electron microscopy. The in vitro dissolution of luteolin raw materials and nanosuspension was compared.[Results] The process parameters and formulation of luteolin nanosuspension were determined by single factor experiment:milling media volume was 1:1,milling speed was 2 500 r/min,the milling time was 4 h,the stabilizer was HPC SL,and the surfactant was TPGs. The optimal formulation of luteolin nanosuspension was optimized by Box-Behnken experimental design:drug concentration was 28.0 mg/mL,stabilizer concentration was 1.5 mg/mL,surfactant concentration was 0.2 mg/mL;The average particle size and Zeta potential of the luteolin nanosuspension prepared according to the optimized formulation were (324.3±21.6) nm and (-31.4±0.9) mV respectively,and a uniform particle distribution could be observed under scanning electron microscopy. The drug dissolution rate of luteolin nanosuspension was significantly higher than that of the luteolin.[Conclusion] In this study,luteolin nanosuspension could significantly improve the drug dissolution rate in vitro,and it was expected to improve oral bioavailability and improve drug treatment. |
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