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| Effect of Didang Decoction on the expression of energy metabolism related factors in human umbilical vein endothelial cells under high glucose and high glucose fluctuating |
| Hits 992 Download times 772 Received:February 15, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.07.25 |
| Key Words
Didang Decoction;high glucose fluctuating;energy metabolism;p-AMPK;PGC-1α;eNOS;caspase-3 |
| Author Name | Affiliation | E-mail | | REN Qiuyue | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | TIAN Hanlin | Tongren City Bijing District Hospital of Traditional Chinese Medicine, Tongren 554300, China | | | CHANG Bai | Tianjin Medical University ZHU Xianyi Memorial Hospital, Tianjin 300134, China | changbai1972@126.com |
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| Abstract
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| [Objective] To observe the effect of Didang Decoction (DDD) on the expression of phosphorylated adenylate activated protein kinase (p-AMPK),peroxisome proliferator activated receptor γ co-activated activator 1α (PGC-1α),endothelial nitric oxide synthase (eNOS),caspase-3 in human umbilical vein endothelial cells (HUVECs) under high glucose and high glucose fluctuation environment,the protective effect of DDD on vascular endothelial cells was discussed.[Methods] HUVECs were cultured in a simulated environment with high glucose and high glucose fluctuation and were intervened by DDD,simvastatin,and metformin respectively. After 48 hours of culture in each group,the protein expressions of p-AMPK,PGC-1α and eNOS were detected by Western blot,the gene content of caspase-3 was detected by real-time PCR (RT-PCR),and the apoptosis rate was detected by flow cytometry.[Results] The expression of p-AMPK,PGC-1α and eNOS in high glucose group and high glucose fluctuation group decreased significantly,and the lowest expression was found in high glucose fluctuation group. The expression of p-AMPK,PGC-1α and eNOS protein were significantly up-regulated after the intervention of DDD,and there was no difference with the intervention of metformin. Caspase-3 gene expression in high glucose group and high glucose fluctuation group were significantly increased,and significantly decreased after intervention with DDD,and there was no difference between them and metformin intervention group.[Conclusion] DDD can promote AMPK phosphorylation,up-regulate the expression of PGC-1α and eNOS proteins,reduce caspase-3 gene level,regulate endothelial cell mitochondrial synthesis and energy metabolism,and reduce endothelial cell apoptosis under high glucose and high glucose fluctuation environment,thus protecting vascular endothelium. |
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