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| Discussion on the effect and mechanism research of Anchang Yuyang Recipe on HT-29 cell inflammation model based on the JAK1/STAT6 pathway |
| Hits 1455 Download times 864 Received:March 14, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.08.21 |
| Key Words
ulcerative colitis;Anchang Yuyang Recipe;JAK1/STAT6 pathway;in vitro experiment |
| Author Name | Affiliation | E-mail | | SUN Dajuan | Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China
The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | | FAN Jingna | The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | | WANG Shuai | Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | | CHI Lili | Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China | chililiyl@163.com |
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| Abstract
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| [Objective] To observe the influence of JAK1/STAT6 pathway on LPS induced HT-29 cell with Anchang Yuyang Recipe (AYR) and to explore the mechanism.[Methods] The experiment was divided into 6 groups:control group,model group,low,medium and high concentration group of Anchang Yuyang Recipe (0.1,1,10 mg/mL),mesalazine group (1 mg/L). The control group was done without any treatment. The model group was induced by LPS. The remaining groups were given corresponding drug intervention after LPS induction. After 24 hours of intervention,the expressions of IL-13 and TFF3 in each group were detected by ELISA and immunofluorescence techniques. The expressions of IL-13,JAK1,STAT6,and TFF3 mRNA and protein in each group were detected by Q-RT-PCR and Western Blot.[Results] Compared with the control group,the IL-8 level in the model group was increased (P<0.01) and the IL-13 level was decreased (P<0.01).Itindicated that the inflammation model was successfully constructed. The levels of JAK1,STAT6 and TFF3 in the model group were also reduced (P<0.05 or P<0.01). Compared with the model group,the secretion levels and the fluorescence intensity of IL-13 and TFF3 increased in each medication group (P<0.05 or P<0.01). The expressions of IL-13,JAK1,STAT6,and TFF3 mRNA and protein were up-regulated (P<0.05 or P<0.01). The secretion levels,fluorescence intensity of IL-13,TFF3and the expression of IL-13,JAK1,STAT6and TFF3 mRNA and protein in the high-concentration group of AnchangYuyang Recipe were higher than those of the medium-concentration group and low-concentration group (P<0.05 or P<0.01). But there was no difference compared with the mesalazine group(P>0.05).[Conclusion] The Anchang Yuyang Recipe relieve the inflammatory injury of HT-29 cell and play a protective role probably by up-regulating secretion of IL-13,activating JAK1/STAT6 pathway,promoting the secretion of mucosal repair factor TFF3. |
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