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| Effect of self-made Gutei Decoction on the balance of Th17/Treg cells in spleen of mice with recurrent abortion |
| Hits 1031 Download times 663 Received:June 16, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.09.23 |
| Key Words
traditional Chinese medicine;Th17;Treg;recurrent spontaneous abortion |
| Author Name | Affiliation | E-mail | | ZHOU Lan | Beijing University of Chinese Medicine, Beijing 100029, China | | | HUANG Haitao | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | XU Cai | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | xucai02@126.com | | ZUO Qianqian | Hebei University of Chinese Medicine, Shijiazhuang 050091, China | | | ZHANG Yunna | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | HUANG Wenling | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | QI Bing | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | BU Xiaoling | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | |
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| Abstract
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| [Objective] To observe the effect of self-made Gutai Decoction on the balance of Th17/Treg cells in spleen of spontaneous abortion model mice and pregnancy outcome.[Methods] The normal pregnancy model was established by CBA/J female mice×BALB/c male mice,and the spontaneous abortion model was established by CBA/J female mice×DBA/2 male mice. The spontaneous abortion pregnant mice were divided into low,medium and high dose Chinese medicine group,dydrogesterone group and spontaneous abortion group. The normal pregnancy group and spontaneous abortion group were given normal saline,while the low,medium and high dose Chinese medicine groups were given 7.125,14.25 and 28.5 g/kg self-made Gutai Decoction respectively,and the dydrogesterone group was given 3.0 mg/kg dydrogesterone solution,all with gavage once a day for 2 weeks. After administration,mice were killed and dissected,the number of uterine embryos was observed and the embryo loss rate was calculated. The content of T helper 17 (Th17) cells and Regulatory T (Treg) cells in spleen and the ratio of Th17/Treg cells were detected by flow cytometry.[Results] 1) Compared to the spontaneous abortion group,the embryo loss rate of the low dose Chinese medicine group was no significant difference (P>0.05). The embryo loss rate of the medium and high dose Chinese medicine and dydrogesterone groups was significantly reduced (P<0.05),and there was no significant difference in embryo loss rate among the medium and high dose Chinese medicine and dydrogesterone groups (P>0.05). 2) Compared to the spontaneous abortion group,the number of Th17,Treg cells and the percentage ratios of Th17/Treg cells of the low dose Chinese medicine group were no significant difference (P>0.05). The number of Th17 cells and the percentage ratios of Th17/Treg cells of the medium and high dose Chinese medicine and dydrogesterone groups was significantly reduced (P<0.05),while the number of Treg cells was significantly increased (P<0.05). There was no significant difference in the number of Th17,Treg cells and the percentage ratios of Th17/Treg cells among the medium and high dose Chinese medicine and dydrogesterone groups (P>0.05).[Conclusion] Self-made Gutai Decoction could reduce Th17/Treg ratio,induce immune tolerance,reduce embryo loss rate and improve pregnancy outcome. |
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