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| Effects of Peiyuan Gushen Fang serum on TGF-beta 1,alpha-SMA and E-cadherin proteins in HBV-transfected HK-2 cells |
| Hits 991 Download times 692 Received:April 27, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.10.20 |
| Key Words
hepatitis B virus associated glomerulonephritis;HK-2 cell;renal interstitial fibrosis;Peiyuan Gushen Fang |
| Author Name | Affiliation | E-mail | | LIU Rong | Taicang Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Taicang 215400, China | | | WANG Yaoguang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | wangyaoguang1012@126.com。 |
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| Abstract
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| [Objective] To explore the mechanism of Peiyuan Gushen Fang in the treatment of renal interstitial fibrosis,the effects of serum containing Peiyuan Gushen Fang on the expression of TGF-beta 1,alpha-SMA and E-cadherin in HBV-transfected HK-2 cells were observed.[Methods] HK-2 cells were cultured in vitro. The amplified and identified plasmids were transfected into HK-2 cells by liposome transfection. According to the experimental purpose,the cells were divided into 7 groups,including Mock group (HK-2 cell group in conventional culture),control group (HK-2 cell group transfected with empty plasmid pHY106),HBV group (HK-2 cell group transfected with empty plasmid and HBV complex (PHY106-HBV);HBV+ACEI group (Benazepril group),HBV+PYGS low group (low concentration group of Peiyuan Gushen Fang),HBV+PYGS middle group (medium concentration group of Peiyuan Gushen Fang);HBV+PYGS high group (high concentration group of Peiyuan Gushen Fang). The experiment was divided into three independent samples (n=3). After 6 hours of HBV transfection,the corresponding drugs in each group were intervened. After 48 hours of drug intervention,the expression levels of HBsAg,HBeAg and TGF-beta 1 in cell lysates were detected by ELISA. Immunocytochemistry was used to detect the expression of alpha-SMA and E-cadherin.[Results] The expression of TGF-beta 1 was down-regulated in all intervention groups of Peiyuan Gushen Fang (P<0.001),and the expression of alpha-SMA was down-regulated significantly (P<0.001),while the expression of E-Cadherin was up-regulated (P<0.05).[Conclusion] Peiyuan Gushen Fang may inhibit the phenotypic transformation of HBV-GN renal tubular epithelial cells by inhibiting the expression of TGF-beta 1. It can up-regulate the expression of E-cadherin in epithelial cells and down-regulate the expression of alpha-SMA in myofibroblasts,to prevent and treat tubulointerstitial fibrosis of HBV-GN. |
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