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| Specific PCR molecular identification of Whitmania pigra |
| Hits 1102 Download times 733 Received:June 20, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.11.21 |
| Key Words
Whitmania Pigra;specific PCR;molecular identification;fluorescence detection |
| Author Name | Affiliation | E-mail | | WANG Wenxiu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GAO Han | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Huanyu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | TAN Wei | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Zhenguo | Mudanjiang YouBo Pharmaceutical Co., Ltd., Mudanjiang 157000, China | lzgjzt@vip.163.com | | TIAN Xiaoxuan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | tian_xiaoxuan@tjutcm.edu.cn |
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| Abstract
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| [Objective] To design the specific identification primers of Whitmania Pigra by searching for single nucleotide polymorphism (SNP) sites,establish specific polymerase chain reaction (PCR) detection technology,and realize rapid identification of Whitmania Pigra and its common adulterants.[Methods] By comparing the cytochrome c oxidase I (COI) sequences of Whitmania Pigra and their common adulterants,searchinging for SNP sites in COI gene sequence of Whitmania Pigra,and then designing specific identification primers.[Results] After the PCR process,the Whitmania Pigra could amplify a 149 bp band,whereas the adulterants can't get the obvious target band. When the amplified product was stained with SYBR Green I dye,the Whitmania Pigra sample showed yellow-green fluorescence under UV light,whereas the adulterants did not show fluorescence.[Conclusion] This method can identify Whitmania Pigra and its common adulterants accurately and quickly,which can provide technical support for the rapid identification of Whitmania Pigra in Chinese medicinal materials markets. |
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