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| Fuming decoction inhibits the activation of lung fibroblast and lung epithelial injury to alleviate bleomycin-induced pulmonary fibrosis through regulating TGF-β1/Smad3 pathway |
| Hits 1090 Download times 646 Received:July 13, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.12.20 |
| Key Words
fuming decoction;TGF-β1;Smad3;pulmonary fibrosis |
| Author Name | Affiliation | E-mail | | YU Honggang | Department of Pulmonary Diseases, Xi'an Hospital of Traditional Chinese Medicine, Xi'an 710012, China | | | FU Dahai | Department of Pulmonary Diseases, Xi'an Hospital of Traditional Chinese Medicine, Xi'an 710012, China | | | YANG Lisheng | Department of Pulmonary Diseases, Xi'an Hospital of Traditional Chinese Medicine, Xi'an 710012, China | | | SONG Chao | Department of Pulmonary Diseases, Xi'an Hospital of Traditional Chinese Medicine, Xi'an 710012, China | | | TANG Junfeng | Department of Traditional Chinese Medicine Pharmacology, Shaanxi University of Chinese Medicine, Xianyang 712000, China | | | QIN Xiaojuan | Department of Respiratory Medicine, Hanzhong People's Hospital, Hanzhong 723000, China | 33911553@qq.com |
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| Abstract
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| [Objective] To explore the mechanism of Fuming decoction in the treatment of pulmonary fibrosis,and to lay a theoretical foundation for its clinical application.[Methods] The effect of Fuming decoction on TGF-β1 signaling pathway was detected by luciferase reporter gene,and its effect on TGF-β1-induced Smad2/3-dependent pathway and non-Smad signaling was detected,qRT-PCR and western blot methods were used to detect the effect of fuming decoction on the levels of ECM-related mRNA and protein and epithelial damage-related mRNA and protein. Finally,mouse lung fibrosis model was established to clarify the therapeutic effect of fuming decoction on pulmonary fibrosis.[Results] Fuming decoction can antagonize TGF-β1/Smad3 signaling,which can not only inhibit TGF-β1-induced alveolar epithelial cell damage,but also reduce TGF-β1-induced fibroblast differentiation,extracellular matrix formation and fibroblast migration. In vivo experiments showed that Fuming decoction could inhibit bleomycin-induced lung injury and even attenuate formed pulmonary fibrosis in mice.[Conclusion] Fuming decoction can alleviate bleomycin-induced pulmonary fibrosis in mice by mediating the TGF-β1/Smad3 pathway,which lays a theoretical foundation for the clinical application of fuming decoction in the treatment of pulmonary fibrosis. |
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