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| Screening,preparation and anti-tumor activity investigation of okicamelliaside in Camellia nitidissima C. W. Chi |
| Hits 1428 Download times 976 Received:October 11, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2020.12.22 |
| Key Words
Camellia nitidissima C. W. Chi;okicamelliaside;near infrared analysis;quality markers;anti-tumor |
| Author Name | Affiliation | E-mail | | CHENG Chuanjing | State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300353, China | | | CONG Longfei | State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300353, China | | | LI Zhenqiang | State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300353, China | | | HAO Erwei | Collaborative Innovation Center of Research on Functional Ingredients from Agricultural Residues, Guangxi University of Chinese medicine, Nanning 530200, China | | | HOU Xiaotao | Collaborative Innovation Center of Research on Functional Ingredients from Agricultural Residues, Guangxi University of Chinese medicine, Nanning 530200, China | | | HOU Yuanyuan | State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300353, China | houyy@nankai.edu.cn | | BAI Gang | State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300353, China | | | DENG Jiagang | Collaborative Innovation Center of Research on Functional Ingredients from Agricultural Residues, Guangxi University of Chinese medicine, Nanning 530200, China | |
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| Abstract
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| [Objective] To investigate the content of okicamelliaside in Camellia nitidissima C. W. Chi from different places by near infrared spectroscopy,and to prepare and investigate its antitumor activity.[Methods] In this experiment,the content of okicamelliaside was determined by HPLC,and its near-infrared spectrum was obtained and established the quantitative model. According to the established model,the quality of okicamelliaside from different areas was evaluated,and high-content samples were selected for the extraction,preparation and activity evaluation.[Results] The established near-infrared detection method was quickly and convenient. The content of okicamelliaside could be determined directly and we can easily screen out high-content samples. Further,bymacroporous adsorption resin separation,HPLC preparation and structure identification,high-purity okicamelliaside was obtained. Cytotoxicity analysis showed that okicamelliaside had a certain activity to inhibit the growth of cancer cells.[Conclusion] This article provides reference quality markers for the quality evaluation of Camellia nitidissima C. W. Chi,and provides a scientific basis for the development and utilization Camellia nitidissima C. W. Chi. |
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