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| Niaodukang improves epithelial-mesenchymal transition of renal tubular epithelial cells in UUO rats by inhibiting p38/ERK MAPK pathway |
| Hits 5421 Download times 1192 Received:August 24, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.01.23 |
| Key Words
Niaodukang;renal fibrosis;mitogen-activated protein kinase signaling pathway |
| Author Name | Affiliation | E-mail | | JIANG Qian | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | WANG Hong | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | WANG Lei | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | KANG Li | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | LI Yanlin | Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, China | li.yan.lin@126.com |
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| Abstract
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| [Objective] To study the effect of Niaodukang on renal function and pathological changes of renal tissue in rats with renal fibrosis,and to explore the mechanism of anti-fibrosis effect of Niaodukang.[Methods] Unilateral ureteral ligation (UUO) was used to replicate the rat renal interstitial fibrosis model. The model rats were divided into control group,model group,positive group (losartan,30 mg/kg,10 mL/kg),Niaodukang 20 mL/kg,10 mL/kg and 5 mL/kg groups according to their body weight. On the second day after the operation,the corresponding test drug was administered by intragastric administration once a day for 21 days. The 24 h urine protein content was measured after the last administration. Serum creatinine (SCr) level was detected. The kidney of the ligation side (left kidney) was dissected and the pathological changes of rat kidney tissue were observed by HE staining,Masson staining was used to observe the degree of fibrosis and score. The expression levels of TGF-β and α-SAM mRNA in kidney were detected by QRT-PCR. The expression level of p38 and ERK protein in kidney was detected by Western blot.[Results] Niaodukang can significantly reduce SCr level (P<0.01) and 24 hour urinary protein level (P<0.01) in UUO model rats. Compared with the model control group,the pathological manifestations in Niaodukang group were reduced to some extent,and the improvement in the 10 mL/kg group was relatively obvious,and the expression of P38 and ERK1/2 protein in kidney was obviously inhibited.[Conclusion] Niaodukang can obviously improve the renal function of UUO model rats and improve the mesenchymal transition of renal tubular epithelial cells on ligation side,which is related to the inhibition of p38/ERK MAPK. |
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