|
| Study of Allicin reverses Cisplatin resistance in human breast cancer MCF-7 cells |
| Hits 1538 Download times 1081 Received:August 19, 2020 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2021.01.26 |
| Key Words
Allicin;breast cancer;Cisplatin;resistance;cell cycle |
| Author Name | Affiliation | | ZHAO Mingzhi | Handan First Hospital, Handan 056000, China | | LI Xue | Handan First Hospital, Handan 056000, China |
|
| Abstract
|
| [Objective] To study the effect of Allicin reverses Cisplatin resistance in human breast cancer MCF-7 cells and related regulatory mechanisms.[Methods] Taking human cisplatin-resistant MCF-7/DDP cells in logarithmic growth phase as test cells,set blank control group (DMSO),Allicin group (20μg/mL),Cisplatin group (6 μg/mL) and combined group (Allicin 20 μg/mL +Cisplatin 6 μg/mL). 48h after given drugs,the proliferation inhibition rate of MCF-7/DDP cells was detected by MTT method,the apoptosis and analysis of cell cycle distribution were detected by Annexin V-FITC staining and PI staining flow cytometry,the expression of Bcl-2,Bax,Cyclin D1,CDK2,CDK4,CDK6 proteins were detected by Western blot.[Results] Compared with Cisplatin group,the proliferation inhibition rate and apoptosis rate of human breast cancer MCF-7 cells in combination group decreased,the ratio of the cells in G0/G1 phase increased,the expression of Bax increased and the expression of Bcl-2 decreased the Bax/bcl-2 ratio increased,the expression of Cyclin D1,CDK2,CDK4,CDK6 proteins decreased,all of the difference above were significant (P<0.05 or P<0.01). Compared with blank group group,the ratio of the cells in G0/G1 phase of Crocin group increased,the expression of Baxincreased and the expression of Bcl-2 decreased,the Bax/bcl-2 ratio increased,the expression of Cyclin D1,CDK2,CDK4,CDK6 proteins decreased,all of the difference above were significant (P<0.05 or P<0.01).[Conclusions] Allicin has pharmacological effects on reversing Cisplatin resistance in human breast cancer MCF-7 cells,and its mechanism may be related to the regulation of apoptosis-related and cycle-related proteins expression,promoting MCF-7/DDP cell apoptosis and blocking the cell cycle process. |
|
|
|
|
|
|
|
