|
| Protective effect of Xinnao Shutong Capsule on primary neurons injured by oxygen-glucose deprivation/reoxygenation |
| Hits 857 Download times 625 Received:November 10, 2020 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2021.02.18 |
| Key Words
Xinnao Shutong Capsule;oxygen-glucose deprivation/reoxygenation;neuron;synapse;MAP-2 |
| Author Name | Affiliation | E-mail | | YUAN Qing | Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese Medicine Pharmacology, State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JIA Zhuangzhuang | Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese Medicine Pharmacology, State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Tong | Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese Medicine Pharmacology, State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Wenjie | Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese Medicine Pharmacology, State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHAI Lijuan | Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese Medicine Pharmacology, State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HU Limin | Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese Medicine Pharmacology, State Key Laboratory of Component-Based Chinese Medicine, Institute of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | hulimin@tjutcm.edu.cn |
|
| Abstract
|
| [Objective] To investigate the effects of Xinnao Shutong (XNST) Capsules on the viability of primary neurons and the expression of microtubule-associated protein-2 (MAP-2) in oxygen-glucose deprivation/reoxygenation cells. [Methods] Newborn SD rats were used to obtain cerebral cortical neurons for primary culture and MAP-2 immunofluorescence identification. Oxygen-glucose deprivation/reoxygenation (OGD/R) injury model was established and CCK-8 was used to detect different concentrations of XNST Capsules on the cell vitality. Immunofluorescence method was used to observe the fluorescence intensity of MAP-2,and the neuronal synaptic length was measured by Image J software. Western blot method to detect MAP-2 protein expression. [Results] The primary culture of neurons was successful,and MAP-2 staining was positive. CCK-8 results showed that XNST Capsules had cytotoxic effects on normal cultured neurons at concentrations of 10,25,and 50 μg/mL (P<0.05). Compared with the OGD/R group,XNST Capsules at 3.125 and 6.25 μg/mL concentration can significantly increase the cell viability (P<0.05). Compared with the control group,the MAP-2 fluorescence intensity and neuron synaptic length in the OGD/R group were significantly reduced (P<0.05). Compared with the OGD/R group,3.125 μg/mL XNST Capsules could significantly increase MAP-2 fluorescence intensity expression and neuronal synaptic length (P<0.05). Western blot results showed that compared with the control group,the MAP-2 protein expression of the model group was significantly reduced (P<0.05). Compared with the model group,XNST Capsules can significantly increase the expression of MAP-2 protein (P<0.05). [Conclusion] XNST Capsules can promote the neuron viability induced by OGD/R,and its mechanism is related to the promotion of cytoskeletal protein MAP-2 expression and synaptic stability. |
|
|
|
|
|
|
|
