|
| Experimental study on the effect of Shenjin Yigu method regulating the proliferation of rabbit chondrocytes to reduce the degeneration of cartilage |
| Hits 834 Download times 530 Received:November 10, 2020 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2021.02.19 |
| Key Words
Shenjin Yigu method;knee osteoarthritis;PTHrP;chondrocyte repair |
| Author Name | Affiliation | E-mail | | WANG Yizhou | Department of Massage, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | | | ZHAO Qiang | Department of Massage, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | ywy0707@sina.com |
|
| Abstract
|
| [Objective] To investigate the effect of Shenjin Yigu method on regulation of PTHrP on proliferation of rabbit chondrocytes and reduction of cartilage degeneration. [Methods] The 50 New Zealand big-eared white rabbits randomly divided into normal group,sham operation group,model group,massage group,sodium hyaluronate group,each group of 10. Improved Hulth method was use to prepare knee osteoarthritis(KOA) rabbit model,and massage group was treated with Shenjin Yigu method during 21 days. Sodium hyaluronate group was treated with articular cavity injection of sodium hyaluronate once a week,for 3 weeks,based on the transmission electron microscope after organelles,scanning electron microscopy observed on the surface of the cartilage degeneration and repair,RT-PCR detection of PTHrP mRNA expression. [Results] RT-PCR method to detect each group of cartilage PTHrP mRNA expression,and there was no significance between sham operation group and normal group in PTHrP mRNA expression (P>0.05). Compared with normal group,there was significance in model group (P<0.05). Compared with model group,the massage group,the sodium hyaluronate group had significant difference (P<0.05). Compared with the massage group,the sodium hyaluronate group had no significant (P>0.05). Compared with the normal group,the massage group,the sodium hyaluronate group had significant difference(P<0.05). Scanning electron microscope showed that the ridges and furrows on the cartilage surface of the model group were obviously disordered,and the collagen fibers were exposed. In the massage group and sodium hyaluronate group,fibers crawled over the cartilage surface,suggesting cartilage repair. Transmission electron microscopy showed that the chondrocytes in the model group were hypertrophy in morphology,abnormal morphology of rough endoplasmic reticulum,multiple membrane pores on the nuclear membrane,disappearance of mitochondria,and fracture of collagen fibers. Chondrocytes in massage group and sodium hyaluronate group had normal morphology and structure,and several autophagosomes could be seen in the cells. [Conclusion] Shenjin Yigu method can regulate the expression of PTHrP by regulating the key protein of cartilage anabolism,slow down cartilage degeneration,promote cartilage repair,and reduce KOA symptoms. |
|
|
|
|
|
|
|
