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| Research on the mechanism of intervention of allergic constitution (TIZHI) by Chinese medicines for expelling wind to relieve superficies based on bioinformatics |
| Hits 1527 Download times 613 Received:December 11, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.03.01 |
| Key Words
allergic constitution (TIZHI);expelling wind to relieve superficies;multi-omics analysis;network pharmacology |
| Author Name | Affiliation | E-mail | | ZHANG Kunlin | CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China
Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, China | | | SUN Pengcheng | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | CHEN Xuemei | National Institute of Traditional Chinese Medicine Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | CHEN Jianxin | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Yan | National Institute of Traditional Chinese Medicine Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Yi | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Ji | National Institute of Traditional Chinese Medicine Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | doctorwang2009@126.com | | WANG Qi | National Institute of Traditional Chinese Medicine Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | wangqi710@126.com |
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| Abstract
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| [Objective] To investigate the molecular mechanism of intervention of allergic constitution (TIZHI) by Chinese medicines for expelling wind to relieve superficies.[Methods] The data of 4 gene sets were composed of the predictive targets of Chinese medicines for expelling wind to relieve superficies, allergic comorbid genes extracted from GWAS catalog database, allergic genes identified by immature dendritic cells (imDEGs) and mature dendritic cells (mDEGs), respectively. They were mutually verified at the pathway level through network pharmacology and multi-omics combined pathway analysis in bioinformatics methods. Meta-analysis was used for P value weighted merging, and the corrected P value was used to screen the pathways that the target proteins may participate in during the allergy process. The hub genes were screened by constructing a protein-protein interaction (PPI) network.[Results] The 545 active ingredients were screened out from 15 Chinese medicines for expelling wind to relieve superficies with anti-allergic effect. The predicted and screened 226 target genes, 427 allergic comorbid genes, 1240 imDEGs, and 743 mDEGs were used in the joint analysis of multi-omics pathways. The 24 multi-omics significant pathways were identified, including 6 allergy-related pathways, namely Interleukin-4 and Interleukin-13 signaling, T cell receptor signaling pathway, Co-stimulation by the CD28 family, cellular response to lipopolysaccharide, TGF-β receptor signaling pathway, antigen processing-cross presentation. Among the 28 target proteins related to the allergy pathways, 11 core targets were identified, namely MAPK8, AKT1, MAPK1, SRC, MAPK14, HSP90AA1, LCK, PARP1, PDPK1, JAK3, and MMP3.[Conclusions] The above-mentioned pathways and targets are associated with the allergy process including the presentation of dendritic cells, T cell receptor signal transduction, Th2 type cytokine secretion, and immune tolerance, revealing the mechanism of the intervention of allergic constitution (TIZHI) by Chinese medicines. Our research provides a methodological framework for exploring the molecular mechanism of intervention of allergic constitution (TIZHI) by Chinese medicines. |
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