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| Discussion on the molecular mechanism of Bushen Cupailuan Decoction in treating ovarian fibrosis in polycystic ovary syndrome rats based on TGF-β/Smads signaling pathway |
| Hits 1122 Download times 560 Received:December 23, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.03.21 |
| Key Words
Bushen Cupailuan Decoction;polycystic ovary syndrome;ovarian fibrosis;TGF-β;Smads |
| Author Name | Affiliation | E-mail | | WANG Xudong | The First People's Hospital of Nantong, Nantong 226001, China | | | ZHOU Jie | The First People's Hospital of Nantong, Nantong 226001, China
Nanjing University of Chinese Medicine, Nanjing 210029, China | | | TAN Yong | Nanjing University of Chinese Medicine, Nanjing 210029, China | | | ZHU Meihong | The First People's Hospital of Nantong, Nantong 226001, China | | | CAO Lin | Affiliated Hospital of Integrated traditional Chinese and Western Medicine Nanjing University of Chinese Medicine, Nanjin 210028, China | | | GAO Jie | The First People's Hospital of Nantong, Nantong 226001, China | zj051051@126.com | | ZHONG Chenxia | The First People's Hospital of Nantong, Nantong 226001, China | |
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| Abstract
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| [Objective] To investigate the therapeutic effect of Bushen Cupailuan Decoction on polycystic ovary syndrome (PCOS) model rats, and to study the molecular mechanism of regulating TGF-β/Smads signaling pathway in ovarian fibrosis.[Methods] Forty SD female rats were randomly divided into the blank group (8 rats) and the Dehydroepiandrosterone (DHEA) group (32 rats). The DHEA group was injected subcutaneously with DHEA (60 mg/kg) daily for 35 days. The successfully modeled rats were randomly divided into the model group and the low-dose, medium-dose and high-dose groups of Bushen Cupailuan Decoction[5.58, 11.16 and 22.32 g/(kg/d)]. Bushen Cupailuan Decoction was prepared with normal saline at appropriate concentration, and was given continuous intragastric administration for 4 weeks, once a day. At the same time, rats in blank group and model group were given the same amount of normal saline for intragastric administration. The estrous cycle of rats was observed by vaginal smear, the effect of Bushen Cupailuan Decoction on ovarian follicle development was observed by Hematoxylin and eosin (HE) staining, ovarian fibrosis was observed by Sirius red staining. The serum estradiol (E2), testosterone (T) and luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were quantified using Enzyme linked immunosorbent assay (ELISA). Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the mRNA expression of TGF-β1, Smad3, Smad7. Western blot was used to detect the protein expression of TGF-β1, p-smad3 and Smad7 in rats.[Results] Compared with the blank group, the rats in the model group lost regular estrus cycle, partial follicular vesicle-like dilation in the ovaries, increased the positive area of rat ovarian fibrosis, and significantly increased serum T and LH. Meanwhile, the expression of TGF-β1, p-Smad3 protein and mRNA increased significantly, while the expression of Smad7 protein and mRNA decreased significantly (P<0.01). Compared with the model group, the medium and high dose groups of Bushen Cupailuan Decoction could effectively improve the estrus cycle of PCOS rats, reduce the formation of follicle expansion, reduce the positive area of ovarian fibrosis (P<0.01), down-regulate the expression of TGF-β1 protein and mRNA in ovary (P<0.05, P<0.01), and significantly down-regulate the expression of p-Smad7 protein and mRNA (P<0.01).[Conclusion] Bushen Cupailuan Decoction can effectively treat PCOS model rats, it may play a role by regulating the expression of TGF-β1, p-Smad3 and Smad7, which are important molecules in fibrotic TGF-β/Smads signal pathway. |
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