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| Effect of rosmarinic acid on PI3K/Akt signal pathway in protecting blood-brain barrier function by hypoxia/reoxygenation injury |
| Hits 1040 Download times 604 Received:September 15, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.03.23 |
| Key Words
rosmarinic acid;blood-brain barrier function;hypoxia/reoxygenation;PI3K/Akt signaling pathway |
| Author Name | Affiliation | E-mail | | CHEN Peng | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | QU Jingtian | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Jingsi | Increasepharm(Tianjin) Institnte Co. Ltd, Tianjin 300385, China | wangjs.521@163.com |
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| Abstract
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| [Objective] To investigate the effect of rosmarinic acid (RA) on blood-brain barrier (BBB) function induced by hypoxia/reoxygenation and its mechanism.[Methods] The changes of cell viability, lactate dehydrogenase leakage rate, transcellular electrical resistance, and fluorescein isothiocyanate glucan permeability were investigated by the monolayer blood-brain barrier model in vitro, and the changes of key signal molecules were analyzed.[Results] Hypoxia/reoxygenation injury can result in decreased cell viability, increased LDH leakage, decreased TEER value, increased FITC-glucan permeability, increased Rh123 accumulation. RA can significantly improve the above injury and maintain BBB function. After administration of RA, the BBB function was significantly improved, and the molecular signal was significantly up-regulated.[Conclusion] RA has protective effect on BBB function in hypoxia/reoxygenation injury, and may be related to its intervention on PI3K/Akt signaling pathway. |
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