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| An experimental study on the inhibition of angiogenesis in prostate cancer by Jianpi Lishi Huayu Prescription based on PI3K/AKT/eNOS signaling pathway |
| Hits 929 Download times 592 Received:November 05, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.04.24 |
| Key Words
Jianpi Lishi Huayu Prescription;prostate cancer;angiogenesis;PI3K/AKT/eNOS signaling pathway |
| Author Name | Affiliation | E-mail | | ZHANG Yao | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | XU Yuemei | Tianjin Hexi District Hospital of Traditional Chinese Medicine, Tianjin 300201, China | | | LI Jiahe | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GUO Shanqi | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | LI Xiaojiang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | JIA Yingjie | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | jiayingjie1616@sina.com |
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| Abstract
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| [Objective] On the basis of the clinical efficacy and preliminary experiments of Jianpi Lishi Huayu Prescription,the function of the prescription and the parts of this prescription in inhibiting angiogenesis of PC-3 tumor-bearing mice with human prostate cancer was explored and its effect on the PI3K/AKT/eNOS signaling pathway were further discussed.[Methods] The 36 male nude rats were selected to establish a tumor-bearing mouse model of human prostate cancer PC-3 cells. They were divided into the control group,Western medicine group,Jianpi Lishi Huayu Prescription group (full-prescription),monarch group (Huangqi,Gancao),minister group (Ciwujia,Buguzhi) and assistant group (Jianghuang,Wangbuliuxing). After 14 days,sacrificed and blood was taken to weigh the tumor and the tumor inhibition rate was calculated. Serum levels of VEGF and eNOS were detected by ELISA. Immunohistochemical method was used to observe the expression of VEGF and ANG1. Western Blot was used to detect the expression levels of PI3K,AKT,p-Akt and eNOS in tumor tissues.[Results] Tumor volume and weight of each administration group was lower than that of the control group (P<0.01). Tumor inhibition rates of each group was 53.95%,41.05%,19.21%,27.37% and 31.32%. Among the different part group of the prescription,the assistant group has better anti-tumor effect,but there is no statistical difference (P>0.05). ELISA showed that the levels of VEGF and ANG1 in each group were lower than control group,Western medicine group and the full-prescription group were significantly decreased (P<0.05,P<0.01). Western Blot showed that the protein expression of PI3K and eNOS in each group was significantly decreased (P<0.05, P<0.01),while the protein expression of AKT showed no significant change (P>0.05),p-AKT showed a decreasing trend (P<0.05),in which the Western medicine group,the full-prescription group and the admixture group showed significant decreases (P<0.01).[Conclusion] Compared with Western medicine,Jianpi Lishi Huayu Prescription has weaker effect on anti-tumor,while Jianpi Lishi Huayu Prescription has a definite inhibitory effect on the angiogenesis of prostate cancer,especially in assistant groupwith the function of removing blood stasis and dispelling syndrome.This may be related to the fact that certain active ingredients in Jianghuang and Wangbuliu can down-regulate PI3K and eNOS proteins,influence AKT phosphorylation,and block PI3K/AKT/eNOS signaling pathways. |
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