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| Preliminary study on the improvement effect and mechanism of Danggui Decoction polysaccharide eextracts on ulcerative colitis |
| Hits 1243 Download times 604 Received:November 30, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.04.25 |
| Key Words
Danggui Decoction polysaccharide extracts;ulcerative colitis;immune regulation;oxidative stress |
| Author Name | Affiliation | E-mail | | GUO Ziyou | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | LIU Jia | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | YU Ling | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | LAN Hai | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | LIU Yujuan | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | WU Qing | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | qwu@vip.Sina.com |
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| Abstract
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| [Objective] To explore the effect and mechanism of Danggui Decoction polysaccharide extracts(DGDPE) in improving ulcerative colitis (UC).[Methods] AUC rat model induced by 2,4,6-Trinitrobenzenesulfonic acid(TNBS) was established to evaluate the effects of DGDPE on UCthrough disease activity index (DAI),colorectal macroscopic injury score,and HE staining colorectal pathology score. RAW264.7 cell model was used to detect the influence of DGDPE on NO production. Spleen cells of normal and UC rats were taken to study the effect of DGDPE on the proliferation of spleen cells under the stimulation with Con-A,and study on mechanism by detecting colorectal tissue oxidative indicators (SOD,MDA,MPO),IL-2 and IFN-γlevels,andspleen cell proliferation.[Results] Rats in the model group had mucus and bloody stools,and the above indicators were significantly different from those in the control group. Compared with the model group,the DAI index,macroscopic injury score,colorectal pathology score,spleen cell stimulation index,IL-2 and IFN-γ levels,MDA content,and MPO activity significantly decreased,and SOD activity significantly increased. Compared with the blank group,DGDPE could significantly increase the production of NO in RAW264.7 cells. But the NO levels of all DGDPE treated groups were significantly lower than that of the positive control stimulated by 1 μg/mL LPS. Compared with the model group,DGDPE could significantly promote the proliferation of normal rat spleen cells and inhibit the proliferation of UC rat spleen cells under the stimulation with Con-A.[Conclusion] DGDPE has an improvement effect on TNBS-induced UC in rats,and its mechanism may be related to the regulation of immunity inhibition and alleviation of oxidative stress. |
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