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| Analysis of volatile components in Asarum heterotropoides by steam distillation and headspace injection gas chromatography-mass spectrometry method |
| Hits 1587 Download times 761 Received:December 30, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.05.23 |
| Key Words
Asarum heterotropoides;volatile compounds;steam distillation;headspace injection;gas chromatography-mass spectrometry |
| Author Name | Affiliation | E-mail | | WANG Wei | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JIA Xiaohua | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GAO Mengyuan | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | OUYANG Huizi | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | hejun673@163.com。 |
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| Abstract
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| [Objective] To analyze and compare the volatile components in Asarum heterotropoides extracted by steam distillation and headspace injection methods.[Methods] The qualitative analysis of the chemical components obtained by the two extraction methods was performed using gas chromatography-mass spectrometry (GC-MS).[Results] A total of 73 volatile components were extracted by the two methods. There are 28 components in common,accounting for 31.46% and 61.55% of the total relative content of the volatile oil extracted by the two methods. Among them,35 chemical components were identified in the volatile oil of Asarum heterotropoides extracted by steam distillation,accounting for 33.75% of the total content of volatile components,mainly phenols,terpenes,and styrenes. The main compound is methyl eugenol (11.97%),3-carene (3.64%),safrole (2.61%),etc. A total of 66 chemical components were identified in the volatile oil of Asarum heterotropoides extracted by the headspace injection method,accounting for 87.16% of the total content of volatile components,mainly phenylpropane,ethers,and terpenes. The main compound is safrole (11.27%),methyl eugenol (10.37%),diethyl phthalate (8.49%),n-hexadecane (7.60%),3,5-dimethoxytoluene (7.51%),2,4,6-Trimethoxytoluene (5.41%),3,4,5-Trimethoxytoluene (4.19%),D-borneol (3.74%),β-pinene (3.30%),α-pinene (3.18%),Eucarvone (2.37%),etc.[Conclusion] The chemical composition and relative content of the volatile oil of Asarum heterotropoides extracted by the two methods are different. Combining the two methods can establish more comprehensive GC-MS information of the volatile components of Asarum heterotropoides. |
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