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| Study of the effect and related mechanism of Tongmai Yangxin Pill on atherosclerosis in ApoE-/- mice |
| Hits 881 Download times 804 Received:March 26, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.06.22 |
| Key Words
atherosclerosis;Tongmai Yangxin Pill;microarray;ApoE-/- mice |
| Author Name | Affiliation | | XIAO Yang | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | LIU Ying | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | XU Yajie | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | LI Guozheng | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | GENG Tong | Tianjin Zhongxin Pharmaceutical Group Co., Ltd., Tianjin 300457, China | | XU Jinpeng | Tianjin Zhongxin Pharmaceutical Group Co., Ltd., Tianjin 300457, China | | WANG Yi | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China |
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| Abstract
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| [Objective] To observe the effect of Tongmai Yangxin Pill treated the arterial plaque in ApoE-/- mice and to explore the mechanism.[Methods] The ApoE-/- mice were randomly divided into four groups,including model group,Tongmai Yangxin Pill 2.0 g/kg group,Tongmai Yangxin Pill 1.0 g/kg group,Tongmai Yangxin Pill 0.5 g/kg group. C57BL/6J mice were control group. ApoE-/- mice were fed with high fat diet to establish atherosclerosis model. One hour after the last administration,the levels of total cholesterol (TC) and triglycerides (TG) in serum were determined by biochemical method. Oil red O staining and HE staining were used to observe the plaque formation and the degree of arterial constriction. Affymetrix mouse genome was used to detect the gene spectrum of mouse artery tissue,and the differential gene analysis,cluster analysis and ingenuity canonical pathway analysis were carried out to explore the pharmacodynamic mechanism of Tongmai Yangxin Pill. RT-PCR and ELISA were used to verify the key proteins or genes.[Results] Compared with the control group,Tongmai Yangxin Pill could effectively improve the formation of large area plaque and significantly improve the narrow artery in the model group. The differential gene enrichment analysis showed that Tongmai Yangxin Pill could inhibit the formation of plaque by affecting PPAR α/RXR α,matrix metalloproteinase signaling pathway and platelet-derived growth factor signaling pathway. The expression of PPAR α gene was significantly up-regulated and the levels of p-NF-κB,TNF-α,IL-6 and IL-1 β were significantly decreased.[Conclusion] Tongmai Yangxin Pill can effectively inhibit the formation of arterial plaque,activate PPAR α and inhibit the expression of inflammatory factors,which is one of the exact ways of drug effect. |
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