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| Effect of Jingui Shenqi Pill on inhibition of apoptosis and autophagy of lung tissue through AMPK/mTOR pathway in rats with chronic obstructive pulmonary disease |
| Hits 868 Download times 591 Received:April 12, 2020 |
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| DOI
10.11656/j.issn.1672-1519.2021.06.23 |
| Key Words
Jingui Shenqi Pill;chronic obstructive pulmonary disease;apoptosis;autophagy;AMPK/mTOR pathway |
| Author Name | Affiliation | E-mail | | LIU Ming | Department of Integrated Traditional Chinese and Western Medicine, Zhuzhou Central Hospital, Zhuzhou 412000, China | | | XIE Li | Department of Integrated Traditional Chinese and Western Medicine, Zhuzhou Central Hospital, Zhuzhou 412000, China | | | PI Miao | Department of Integrated Traditional Chinese and Western Medicine, Zhuzhou Central Hospital, Zhuzhou 412000, China | | | WANG Ling | Department of Integrated Traditional Chinese and Western Medicine, Zhuzhou Central Hospital, Zhuzhou 412000, China | 1065984389@qq.com |
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| Abstract
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| [Objective] To explore the effect of Jingui Shenqi Pills on chronic obstructive pulmonary disease (COPD) and its possible mechanism.[Methods] Forty SD rats were randomly divided into control group,COPD group,Jingui Shenqi Pills 3.7 g/kg group,Jingui Shenqi Pills 7.4 g/kg group and dexamethasone group,8 rats in each group. Except for the control group,the rats in the remaining groups were established with COPD rats by smoking combined with intratracheal lipopolysaccharide (LPS) method. Each group was given corresponding drug treatment. AniRes2005 animal lung function analysis system was used to detect rat lung function. ELISA kits were used to detect the levels of IL-1β,TNF-α,IL-6 and IL-17. HE staining was used to detect lung histopathological changes. TUNEL staining was used to detect the cell apoptosis of lung tissue. Western blot was used to detect the autophagy and AMPK/mTOR pathway related protein expression.[Results] Compared with the control group,the lung function of rats in COPD group was reduced,and the levels of IL-1β,TNF-α,IL-6 and IL-17 were significantly increased (P<0.05),inflammation score,apoptosis rate and LC3Ⅱ/LC3Ⅰ,Beclin1,p-AMPK/AMPK protein expression levels were significantly increased (P<0.05),p62 and p-mTOR/mTOR protein expression levels were significantly reduced (P<0.05);compared with COPD group,pulmonary function was increased in the Jingui Shenqi Pills 3.7 g/kg group,Jingui Shenqi Pill 7.4g/kg group and the dexamethasone group,and the levels of IL-1β,TNF-α,IL-6 and IL-17 were significantly reduced (P<0.05),inflammation score,apoptosis rate and LC3Ⅱ/LC3Ⅰ,Beclin1,p-AMPK/AMPK protein expression levels were significantly reduced (P<0.05),p62 and p-mTOR/mTOR protein expression levels were significantly increased (P<0.05).[Conclusion] Jingui Shenqi Pills may inhibit the apoptosis and autophagy of lung tissue through the AMPK/mTOR pathway,and delay the progression of chronic obstructive pulmonary disease in rats. |
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