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| Codonopsis pilosula polysaccharides regulate NF-κB signaling pathway to alleviate T cell immune disorders and airway inflammation in rats with chronic obstructive pulmonary disease |
| Hits 1254 Download times 537 Received:April 08, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.06.24 |
| Key Words
codonopsis pilosula polysaccharides;chronic obstructive pulmonary disease;syndrome of qi deficiency of spleen and lung;T cell immunity;airway inflammation;NF-κB signal pathway |
| Author Name | Affiliation | E-mail | | LIN Xiaoling | Department of Pharmacy, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, China | | | FANG Cao | Department of Pharmacy, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, China | tjdaiyan@126.com | | KE Weiqiang | Department of Pharmacy, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, China | |
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| Abstract
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| [Objective] To study the Codonopsis pilosula polysaccharides by regulating NF-κB signaling pathway to alleviate T cell immune disorder and airway inflammation in rats with Chronic obstructive pulmonary disease (COPD).[Methods] The 50 SD rats were randomly selected from 60 SD rats to establish the COPD lung spleen qi deficiency syndrome model,and the remaining 10 were recorded as the control group. The COPD lung and spleen qi deficiency syndrome rat model was established by lipopolysaccharide (LPS) and smoke with the infusion of sennata leaves. The successfully modeled rats were randomly divided into 5 groups. In the dexamethasone group,1.95 mg/kg dexamethasone was given normal saline according to the body weight 1 mL/100 g by gavage. Codonopsis pilosula polysaccharides high,medium and low dose groups were given 200,100 and 50 mg/kg of Codonopsis pilosula polysaccharides respectively,which was dissolved in normal saline according to the body weight 1mL/kg by gavage. The rats in the model group and the control group were given 1 mL/kg normal saline. Once a day for 30 days,the levels of CD3+,CD4+,CD4+/CD8+ in peripheral blood of rats were detected by flow cytometry. Inflammatory cell count in lavage fluid of lung tissue was detected by Giemsa staining (Giemsa). Lung lesions in rats was detected by HE staining. The mRNA relative expression levels of nuclear transcription factor κB (NF-κB) and nuclear factor κB inhibitory protein α (IκBα) in rat lung tissues were measured by real-time quantitative polymerase chain reaction (qRT-PCR). NF-κB and IκBα binding levels in lung tissue were detected by immunoprecipitation. NF-κB and DNA in rat lung tissue were detected by electrophoretic mobility shift assay (EMSA). The protein expression levels of nuclear NF-κB,cytoplasmic NF-κB,IκBα and p-IκBα in rat lung tissues were detected by Western Blot (WB).[Results] After treatment,the peripheral blood CD3+,CD4+,CD4+/CD8+ levels of the dexamethasone group and the Codonopsis pilosula polysaccharides high,medium and low dose groups were higher than that in the model group (P<0.05). The number of macrophages,lymphocytes and neutrophils in the model group were higher than that in the control group (P<0.05),the dexamethasone group and Codonopsis pilosula polysaccharides high,medium and low dose groups were lower than the model group (P<0.05). In the model group,the lung tissues showed severe inflammatory cell infiltration,and the air tube wall was deformed and thickened,the dexamethasone group and Codonopsis pilosula polysaccharides high,medium and low dose groups all improved. The results of immunocoprecipitation showed that I kappa B α and NF-kappa B could be combined,and the model group had the least binding rate of IκBα and NF-κB,a little more in the low dose group of Codonopsis polysaccharide,more dexamethasone group and medium dose group,more in the high dose group of dangshen polysaccharide,and the most in the control group.,IκBα mRNA,cytoplasmic NF-κB protein and IκBα protein relative expression levels showed:the model group is lower than the control group (P<0.05),the dexamethasone group and the Codonopsis pilosula polysaccharides high,medium and low dose groups were higher than the model group (P<0.05).[Conclusion] Codonopsis pilosula polysaccharides can inhibit T-cell immune disorder in rats with COPD lung and spleen qi deficiency syndrome,reduce airway inflammation and lung tissue lesions. It may be related to the inhibition of NF-κB signaling,down-regulation of NF-κB mRNA and nuclear NF-κB protein expression levels,up-regulation of IκBα mRNA and protein expression levels,up-regulation of cytoplasmic NF-κB protein expression level,and inhibit NF-κB nuclear displacement,inhibition of p-IκBα. |
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