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| Mechanism of anti-lymphangiogenesis related factors by curcumin in gastric cancer |
| Hits 824 Download times 603 Received:April 19, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.06.25 |
| Key Words
curcumin;SMYD3;VEGF-C;VEGF-D |
| Author Name | Affiliation | E-mail | | LIU Honggen | Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YANG Peiying | Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Xiaojiang | Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIA Yingjie | Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | jiayingjie1616@sina.com |
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| Abstract
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| [Objective] To investigate the molecular mechanism of anti-lymphangiogenesis related factors by curcumin in gastric cancer.[Methods] The MTT was used to test the mobility of MGC803 cell lines. The SMYD3(SET and MYND domain-containing protein 3),VEGF(vascular endothelial growh factors)-C and VEGF-D mRNA and protein expression level were detected by quantitative real-time reverse transcription PCR and Werstern Blot.[Results] The expression of VEGF-C and VEGF-D mRNA and protein in MGC803 cell line were significantly lower after treating with SMYD3 inhibitor BCI-121 in MGC803 cell line. In low expression of SMYD3 cell line (MGC803-sh-SMYD3),the expression of VEGF-C and VEGF-D mRNA and protein were significantly lower than MGC803 cell line. MTT showed that 50 μmol/L curcumin had the strongest inhibitory effects,and the mRNA and protein expression levels of VEGF-C and VEGF-D were significantly eliminated by curcumin. Compared with treatment with curcumin alone,the expression of VEGF-D mRNA and protein were significantly decreased in MGC803 with treating both BCI-121 and curcumin. Furthermore,we found the expression of VEGF-D mRNA and protein in MGC803-sh-SMYD3 were decreased compared with MGC803 cell line when treated with curcumin,while not VEGF-C expression.[Conclusion] It suggested that curcumin might exert anti-VEGF-D in gastric cancer by inhibition of SMYD3 signaling. |
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