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| Effect of Salvianolic acid B on PI3K/Akt pathway on STZ induced apoptosis of rat islets cells |
| Hits 956 Download times 614 Received:January 27, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.06.26 |
| Key Words
Salvianolic acid B;islets;apoptosis;Akt |
| Author Name | Affiliation | | YUAN Jie | Department of Endocrinology, Tianjin Fifth Central Hospital, Tianjin 300450, China | | WANG Su | Department of Endocrinology, Tianjin Fifth Central Hospital, Tianjin 300450, China | | JIANG Yunsheng | Department of Endocrinology, Tianjin Fifth Central Hospital, Tianjin 300450, China | | HU Zhonghui | Department of Endocrinology, Tianjin Fifth Central Hospital, Tianjin 300450, China |
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| Abstract
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| [Objective] To study whether salvianolic acid B (SalB) exert protective effects on damaged rat islets.[Methods] The purified rat islets were divided into 5 groups:control group,streptozocin (STZ)-treated group,low dose of SalB (STZ+SalB15),medium (STZ+SalB30) and high dose of SalB (STZ+SalB60) groups. The pancreatic islets in Control group were treated with citric acid-sodium citrate buffer solution. The islets in STZ-treated group were treated with 8 μmol/L of STZ for 4 h and then replaced with ordinary complete medium. The STZ+SalB groups were treated with STZ for 4 h. The STZ solution was removed and islets were treated with different concentrations of SalB(15 μmol/L,30 μmol/L,60 μmol/L) for 72 h. The apoptosis of each group of islets was detected by TUNEL staining. Insulin levels in the supernatant of islets were detected by ELISA. Then,islets were directly treated with low(15 μmol/L),medium (30 μmol/L) and high (60 μmol/L) doses of SalB,and the ratio of live cells/late apoptotic cells were detected by FDA/PI double staining. Western blot was used to detect the expression of PI3K,Akt and p-Akt protein in each group of islets.[Results] Compared with Control group,TUNEL positive rate of islets increased significantly in STZ group(P<0.01). Medium and high doses of SalB significantly decreased the TUNEL positive rate by comparing with STZ group(P<0.01),indicated that medium and high doses of SalB effectively inhibited STZ-induced apoptosis of rat islet cells. Compared with Control group,there was no significant difference in the percentage of FDA-positive (live cells) in the low,medium and high doses of SalB groups (P>0.05),indicated that the low,medium and high doses of SalB have no damaging effect on normal islet cells. Compared with Control group,insulin release of islets significantly decreased in STZ group (P<0.01). Compared with STZ group,medium and high doses of SalB promoted insulin release of the islets (P<0.05). Compared with STZ group,the medium and high doses of SalB increased the levels of PI3K protein,and had no effects on the expression of Akt protein,whereas significantly increased the levels of p-Akt.[Conclusion] SalB inhibits STZ-induced apoptosis of islet cells,and SalB itself has no damage on islet cells. The mechanism of its inhibition of apoptosis may be related to the activation of PI3K/Akt signaling pathway by enhancing Akt phosphorylation. |
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