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| Study on the mechanism of Qiankundan Ⅵ in the treatment of diabetic kidney disease based on network pharmacology and molecular docking |
| Hits 769 Download times 830 Received:March 22, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.07.22 |
| Key Words
Qiankundan Ⅵ;diabetic kidney disease;network pharmacology;molecular docking;active ingredient;target point;signal pathway |
| Author Name | Affiliation | E-mail | | CHEN Zhiyong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin Beichen District Hospital of Traditional Chinese Medicine, Tianjin 300400, China | | | ZHANG Jinping | Tianjin Beichen District Hospital of Traditional Chinese Medicine, Tianjin 300400, China | | | LI Lianrui | Tianjin Beichen District Hospital of Traditional Chinese Medicine, Tianjin 300400, China | | | LIU Jing | Tianjin Beichen District Hospital of Traditional Chinese Medicine, Tianjin 300400, China | | | LI Tianxiang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | litianxiang612@sina.com |
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| Abstract
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| [Objective] To explore the mechanism of Qiankundan Ⅵ in preventing and treating diabetic kidney disease (DKD) by the method of network pharmacology.[Methods] The chemical components and corresponding targets of Qiankundan Ⅵ were obtained based on TCMSP database,and disease targets of DKD were searched by GeneCards and OMIM database. The key action targets of Qiankundan Ⅵ for prevention and treatment of DKD were obtained by mapping drugs and disease targets. The protein-protein interaction (PPI) network of key targets was constructed by using String database. The core targets and functional modules of Qiankundan Ⅵ for prevention and treatment of DKD were screened by using Cytoscape software. The chemical components and core proteins of Qiankundan Ⅵ were linked by using autodock software. GO and KEGG pathway enrichment analysis was carried out on key targets,and the potential action mechanism of Qiankundan Ⅵ on DKD was analyzed.[Results] There were 103 kinds of effective components and 137 targets in Qiankundan Ⅵ. Quercetin,kaempferol,β-sitosterol and other components with more predicted targets were obtained,including 128 key targets and 6 molecular functional modules. The results of molecular docking showed that the average binding energy between the chemical components of Qiankundan Ⅵ and the target protein was-6.43 kcal/mol,and the binding energy between quercetin and PSGT2 was the highest. The results of GO and KEGG pathway enrichment analysis showed that the prevention and treatment of DKD by Qiankundan Ⅵ was related to the AGE-RAGE signal pathway,Th17 cell differentiation,IL-17 signal pathway,calcium signal pathway,TNF signal pathway and HIF-1 signal pathway in diabetic complications.[Conclusion] Qiankundan Ⅵ can prevent and treat DKD through the synergistic effect of multi-components,multi-targets and multi-signal pathways,which provides an important scientific basis for further clinical and experimental research of Qiankundan Ⅵ. |
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