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| Effect of triptolide on the expression of T-bet/GATA3 and CXCL10/CXCR3 in rheumatoid arthritis patients |
| Hits 787 Download times 501 Received:February 27, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.08.25 |
| Key Words
triptolide;rheumatoid arthritis;T-bet;GATA3;CXCL10;CXCR3 |
| Author Name | Affiliation | E-mail | | WANG Lixin | Tianjin Hospital, Tianjin 300211, China | | | ZHANG Tong | Tianjin Hospital, Tianjin 300211, China | zttsmkx@163.com | | LIU Xiuchan | Tianjin Hospital, Tianjin 300211, China | | | WANG Yi | Tianjin Hospital, Tianjin 300211, China | |
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| Abstract
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| [Objective] To investigate the effects of triptolide(TP) on the expression of T-bet/GATA3, CXCL10 and CXCR3 in rheumatoid arthritis (RA) patients.[Methods] L929 cells were used to investigate the cytotoxicity of TP and determine the concentration of experimental drugs. The cell toxity of TP and the proliferation of PBMC were analyzed by CCK-8. The subpopulaions of Th, Th1 and Th2 from RA patients' peripheral mononuclear cells (PBMC) together with the expression of CXCR3 receptor were detected by flow cytometry. The expression level of IFN-γ, IL-17/IL-17A, TNF-α, IL-4, IL-6, IL-10 and CXCL10 secreted by PBMC were demonstrated by Luminex. mRNA expression of T-bet, GATA3, CXCL10 and CXCR3 were analyzed by RT-qPCR.[Results] When the concentration was less than 25 nmol/L and cultured for 48 h, TP had no significant cytotoxicity to L929. Within this concentration range, when the concentration of TP was only 5 nmol/L, it showed a significant effect on inhibition of PBMC proliferation(P<0.05). The subpopulations of Th, Th1 and CXCR3 expression were inhibited by TP with dose dependent manner (P<0.05), except Th2. The ratio of Th1/Th2 was lower after TP treatment. The expression levels of IL-4, IL-10 and IFN-γ, IL-6, TNF-α, IL-17/IL-17A, CXCL10 were all reduced (P<0.05). Additionally, only CXCL10 expression was decreased (P<0.05), while the expression levels of T-bet, GATA3 and CXCR3 had no significant difference(P>0.05).[Conclusion] TP might inhibit RA immune reaction through supressing mutiple cytokines from Th1 and Th2 cells with a non-spicific way, however TP could down regulate CXCL10 and CXCR3 expression significantly except T-bet or GATA3. |
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