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| Salvia polyphenolate reduces the intestinal ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress |
| Hits 709 Download times 449 Received:March 28, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.09.22 |
| Key Words
Salvianolate;intestinal ischemia-reperfusion;endoplasmic reticulum stress;inflammation;apoptosis |
| Author Name | Affiliation | | LI Guibin | Department of Surgery, Handan General, Jizhong Energy Fengfeng Group Hospital, Handan 056002, China |
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| Abstract
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| [Objective] To investigate the protective effect of Salviaolate (SAL) on intestinal tissue after ischemia/reperfusion (I/R) in rats and explore its mechanism.[Methods] 144 rats were randomly divided into sham operation group (Sham group),I/R group,SAL low (10 mg/kg),medium (20 mg/kg),high (40 mg/kg) dose group and Extract of Ginkgo Biloba (EGB) group (positive control group, 3.15 mg/kg);the rat models with intestinal I/R injury were made by clipping superior mesenteric artery for 60 mins. 30 mins before surgery,the drugs were given by intraperitoneal injection. 120mins later,the water content of intestinal tissue was calculated by dry/wet method;the mesentery pathological examination and apoptosis observation were performed by hematoxylin-eosin (HE) staining method and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method respectively,and the injury score (Chiu's score) and apoptosis index (AI) were calculated;the levels of inflammatory factors[C reaction protein (CRP),tumor necrosis factor-α (TNF-α),interleuk-1β (IL-1β)] in intestinal tissue were detected by enzyme linked immunosorbent assay (ELISA) method;the expression of endoplasmic reticulum stress-related proteins[glucose regulated protein 78 (GRP 78),Jun N-terminal kinases (p-JNK),C/Ebp-homologous protein (CHOP)],nuclear factor kappa-B (NF-κB),Cleved Caspase-3 were detected by Western blot method.[Results] Compared with I/R group,the water content in Salvianolate medium,high dose group and EGB group were significantly decreased (P<0.05 or P<0.01);the mesenteric cell detachment,lamina propria separation,inflammatory cell infiltration and other diseases,and cell apoptosis were significantly improved;the Chiu's score and AI were significantly reduced (P<0.05 or P<0.01);the level of CRP,TNF-α,IL-1β were significantly decreased (P<0.01);the expression of RP78,p-JNK,CHOP,NF-κB,Cleved Caspase-3 were significantly down-regulated (P<0.05 or P<0.01). Compared with EGB group,the Chiu's score and AI were significantly reduced (P<0.05);the level of CRP,TNF-α were significantly decreased(P<0.05 or P<0.01);the expression of RP78,CHOP,NF-κB,Cleved Caspase-3 were significantly down-regulated (P<0.05 or P<0.01).[Conclusion] SAL has protective effect on intestine I/R injury in rats;which mechanism may be related to SAL inhibiting the endoplasmic reticulum stress pathway,thereby inhibiting inflammation and apoptosis. |
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