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| Regulatory effect of Shenyan Kangfu Tablet on short-chain fatty acids in feces of diabetic nephropathy mice |
| Hits 1285 Download times 645 Received:August 10, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.10.21 |
| Key Words
short-chain fatty acids;propionic acid;diabetic nephropathy;GC-MS/MS;intestinal bacteria |
| Author Name | Affiliation | E-mail | | WANG Xiaoli | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Chunxia | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHEN Qian | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Jia | Tianjin Tongrentang Group Co., Ltd., Tianjin 300385, China | | | WANG Tao | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHEN Xiaopeng | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | xpchen@tjutcm.edu.cn |
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| Abstract
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| [Objective] To study the changes in the content of short-chain fatty acids in the feces of the intestinal flora metabolites of diabetic nephropathy mice interfered by Shenyan Kangfu Tablets, and explain the mechanism of action of Shenyan Kangfu Tablets in improving diabetic nephropathy from the perspective of intestinal microecological metabolites. [Methods] To detect the level of short-chain fatty acids in the feces of db/db mice based on the GC-MS/MS system and standard curve method, and conduct statistical analysis and correlation analysis with changes in intestinal flora. Taking db/db gene-deficient mice as the model mice for diabetic nephropathy, and taking m/m mice as the normal group, the db/db mice were randomly divided into model group, metformin group and high and low dose groups of Shenyan Kangfu Tablets. After the three months administration, the feces of each mouse were collected and recorded. The blood glucose and body mass of mice were monitored regularly before and after modeling and medication. [Results] Under the conditions of gas chromatography-mass spectrometry with a temperature program and a carrier gas flow of 1.2 mL/min, acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, caproic acid and valeric acid were effectively separated from the stool. Compared with the model group, the content of short-chain fatty acids in each administration group showed a downward trend, and the content of propionic acid was statistically significant in the model group compared with each administration group (P<0.05). This result mutually confirmed by the decrease in the relative abundance of Bacteroides in the intestinal flora. [Conclusion] This experiment established an effective method to determine the content of short chain fatty acids in the feces of mice, and found that the content of propionic acid in short chain fatty acids of db/db mice after Shenyan Kangfu Tablets intervention showed a downward trend, and present research points out that propionic acid plays an important role in energy metabolism and inflammation. The results provide data support for the mechanism of Shenyan Kangfu Tablets in the treatment of diabetic nephropathy, and provide a reference for the research and development of drugs for the treatment of diabetic nephropathy. |
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