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| Effect and mechanism of formulae Bupleuri Radix Granule on hypertriglyceridemia in fructose-fed rats |
| Hits 1237 Download times 646 Received:June 10, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.10.22 |
| Key Words
formulae Bupleuri Radix Granule;high triglycerides;hypolipidemic;adipose tissue;AMPK/SIRT1/PGC1α |
| Author Name | Affiliation | E-mail | | CHEN Yuwei | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XI Yumeng | Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing Medical University, Chongqing 400016, China | | | LI Pan | Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing Medical University, Chongqing 400016, China | | | LUO Xianqin | Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing Medical University, Chongqing 400016, China | | | LI Wei | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Jianwei | Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing Medical University, Chongqing 400016, China | wjwcq68@163.com |
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| Abstract
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| [Objective] To observe the lipid-lowering effect of formulae Bupleuri Radix granules on fructose-induced hypertriglyceridemia rats and explore its potential pharmacological mechanisms. [Methods] Forty Sprague Dawley(SD) rats were randomly divided into normal control group, fructose-induced group and formulae Bupleuri Radix group. Fructose-induced group rats were treated with 10% fructose water(W/V, prepared daily), while formulated Bupleuri Radix groups were intragastricly administrated with different doses of formulae Bupleuri Radix Granules (including high, middle and low dose). Oral glucose tolerance test (OGTT) was performed at the end of week 4. Blood was collected for detecting the plasma biochemical indexincluding triglyceride(TG) and glucose. Moreover, epididymal white adipose tissue(e-WAT) and the liver were collected. The morphological changes of e-WAT were observed by haematoxylin and eosin (HE) staining, meanwhile, the expression of related genes and proteins of e-WAT orliver were detected by real-time quantitative polymerase chain reaction(qRT-PCR) and Western-blot analysis. [Results] Formulae Bupleuri Radix Granules significantly alleviated fructose-induced increase of plasma TG(P<0.05) and improved glucose tolerance, while there is no significant change in hepatic TG among these groups. Moreover, there were no obvious effect of formulae Bupleuri Radix Granules on the increase of lipid synthesis transcription including gene carbohydrate response element binding protein(CHREBP), fatty acid synthesis gene[fatty acid synthase (FASN), stearoyl-CoA desaturase-1 (SCD1) and fatty acid metabolism gene recombinant human fibroblast growth factor-21 (FGF21)]. Meanwhile, formulae Bupleuri Radix granules caused no significant changes of the reduction of fatty acid oxidation gene such as peroxisome proliferator activated receptor-α (PPAR-α), carnitine palmitoyl transferase-1α (CPT1α) and peroxisome proliferator-activated receptor-γ coactivator 1α(PGC-1α)in the liver of high fructose-fed rats. Additionally, formulae Bupleuri Radix Granules could obviously reduce the cell volume of eWAT, and it also promote the phosphorylation of AMP-dependent protein kinase (AMPK), increase the expression of PGC-1α and SIRT1 in eWAT of fructose-fed rats. [Conclusion] Formulae Bupleuri Radix Granules ameliorated hypertriglyceridemia through strengthening AMPK/SIRT1/PGC-1α signal transduction in eWAT of fructose-fed rats. |
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