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| Effect of extract of Lycopus lucidus Turcz protect the cell from neuronal ischemic injury model by targeting NDRG2 |
| Hits 1171 Download times 677 Received:July 10, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.10.23 |
| Key Words
cerebral ischemia injury;PC12;Lycopus lucidus Turcz extract;NDRG2;proliferation;apoptosis |
| Author Name | Affiliation | | WANG Jing | Department of Neurology, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China | | ZHUO Sisi | Department of Neurology, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China | | QIAO Nan | Department of Neurology, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China |
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| Abstract
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| [Objective] To study the effect and potential molecular mechanism of Lycopus lucidus Turcz extract on nerve cell damage caused by low glucose and hypoxia. [Methods] PC12 cells were used to establish cerebral ischemia injury model. Then the model cells were treated with Lycopus lucidus Turcz extract of 0.1, 1 and 10 mg/mL. OD value of PC12 cells was measured by MTT assay. The expression levels of NDRG2, Cyclin D1, p21, Bax and Bcl-2 protein was detected by Western blot, and apoptotic rate was determined by flow cytometry. [Results] Compared with the control group, both 1 mg/mL and 10 mg/mL Lycopus lucidus Turcz extract increased OD value of PC12 cells in the model group, increased expression of CyclinD1 and Bcl-2, decreased expression of NDRG2, p21 and Bax, promoted cell proliferation and inhibited apoptosis. Inhibition of NDRG2 promoted proliferation and inhibited apoptosis of PC12 cells in the model group. Overexpression of NDRG2 reversed the effects of Lycopus lucidus Turcz extract on proliferation and apoptosis of PC12 cells in the model group.[Conclusion] Lycopus lucidus Turcz extract promoted the proliferation and inhibited the apoptosis of cerebral ischemia injury model cells PC12 by inhibiting NDRG2. Lycopus lucidus Turcz extract may have potential therapeutic effects on cerebral ischemic injury of nerve cells. |
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