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| Resveratrol synergizes the inhibitory effect of 5-fluorouracil in colon cancer by suppressing PI3K/Akt signaling pathway |
| Hits 1395 Download times 602 Received:June 15, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.10.24 |
| Key Words
resveratrol;colon cancer;5-fluorouracil;PI3K/Akt pathway |
| Author Name | Affiliation | E-mail | | CUI Yonghe | Affiliated Hospital of Hubei University of Arts and Science, Xiangyang Central Hospital, Xiangyang 441021, China | | | SHEN Xianmin | Affiliated Hospital of Hubei University of Arts and Science, Xiangyang Central Hospital, Xiangyang 441021, China | jfc55555@163.com |
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| Abstract
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| [Objective] To observe the effects of resveratrol (RES) on the chemotherapeutic sensitivity of 5-fluorouracil(5-FU) in colon cancer and PI3K/Akt signaling pathway.[Methods] SW620 cells were cultured in vitro. CCK8 assay was performed to detect the cell viability. Cell colonies were measured by inverted microscope. SW620 cell apoptotic rate was determined using flow cytometry. The expression levels of apoptosis related proteins including Cle-caspase 9, Cle-caspase 7 and Cle-PARP and proteins in PI3K/Akt signaling pathway were detected by Western Blot. [Results] Compared with control group, the cell viability, colonies and the expression levels of p-p85, p-110β, p-PDK1 and p-Akt in RES group, 5-FU group and RES+5-FU group were significantly decreased, whereas cell apoptosis and the expression levels of Cle-caspase 9, Cle-caspase 7 and Cle-PARP were greatly increased (P<0.05). Compared with RES group and 5-FU group, the cell viability, colonies and the expression levels of p-p85, p-110β, p-PDK1 and p-Akt in RES+-FU group were significantly decreased, whereas cell apoptosis and the expression levels of Cle-caspase 9, Cle-caspase 7 and Cle-PARP were greatly increased (P<0.05). Moreover, PI3K inhibitor (LY294002) promote the inhibitory effect of RES and 5-FU combination on the growth of SW620 cells, whereas PI3K overexpression inhibits it. [Conclusion] RES and 5-FU synergized the chemotherapeutic effect of colon cancer through the suppression of PI3K/Akt signaling pathway. |
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