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| Effect of electroacupuncture at Renying and Taichong on kidney tissue Foxp3 and RORγt in spontaneous hypertension rats model |
| Hits 744 Download times 435 Received:May 20, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.11.13 |
| Key Words
acupuncture;prehypertension;FoxP3;RORγt |
| Author Name | Affiliation | | ZHANG Lili | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China
Tianjin Key Laboratory of Acupuncture and Moxibustion Science, Tianjin 300193, China | | DU Kai | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China | | WANG Yang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China | | WANG Wenhui | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China | | ZHAO Mengxiong | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China | | SHEN Yan | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China
Tianjin Key Laboratory of Acupuncture and Moxibustion Science, Tianjin 300193, China
Tianjin Acupuncture and Moxibustion Research Institute, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the effect of "Huoxue Sanfeng" acupuncture on the protein and mRNA expression of FoxP3 and RORγt in kidney tissue of prehypertensive rats. [Method] Sixteen 4-week-old spontaneously hypertensive rats were randomly divided into a model group (SHR group) and an electro-acupuncture group (EA+SHR group),8 rats in each group. 8 WKY rats served as a blank control group. In EA+SHR group,acupuncture were performed at Renying and Taichong bilaterally,once a day for 2 weeks. The Softron non-invasive blood pressure measurement was used to measure the systolic blood pressure before intervention,1 week and 2 weeks after EA treatment. HE staining was used to detect the morphology of rats renal. Western blot and PCR technology were applied to assessed the FoxP3,RORγt protein and mRNA expression. [Result] The level of systolic blood pressure in SHR rats was significantly higher than that in WKY group (P<0.05). After two weeks of intervention,the level of systolic blood pressure in EA+SHR group was significantly lower than that of SHR group (P<0.05). The expression of RORγt mRNA in kidney tissue of SHR group was significantly higher than that of WKY group (P<0.05),FoxP3 protein and mRNA expression,as well as the FoxP3/RORγt ratio was significantly lower than that of WKY group (P<0.05). The renal tissue RORγt mRNA expression of EA+SHR group was significantly lower than that of SHR group (P<0.05),FoxP3 protein expression and FoxP3/RORγt ratio were significantly higher than that of SHR group (P<0.05). There was no obvious change of renal morphology after EA intervention. [Conclusion] "Huoxue Sanfeng" acupuncture can regulate the expression of FoxP3 and RORγt,and the antihypertensive effect of EA on prehypertensive rats may be related to regulating the balance of FoxP3 and RORγt. |
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