|
| Effect of Ganlu Xiaoke Capsule on JAK2/STAT3 signal pathway in rat model of diabetic nephropathy |
| Hits 719 Download times 479 Received:June 01, 2021 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2021.11.17 |
| Key Words
Ganlu Xiaoke Capsule;streptozotocin;diabetic nephropathy |
| Author Name | Affiliation | E-mail | | LEI Hong | Department of Pharmacy, Inner Mongolia People's Hospital, Hohhot 010017, China | | | ZHANG Ye | Department of Medicine, Hetao College, Bayannur 015000, China | | | XIAO Weili | Department of Clinical Laboratory, Inner Mongolia People's Hospital, Hohhot 010017, China | | | SU Hongquan | Department of Traditional Chinese Medicine, Inner Mongolia People's Hospital, Hohhot 010017, China | | | YANG Hongxin | Department of Pharmacy, Inner Mongolia People's Hospital, Hohhot 010017, China | 1154402768@qq.com |
|
| Abstract
|
| [Objective] To study the therapeutic effect of Ganlu Xiaoke Capsule on diabetic nephropathy. [Methods] The 70 male SD rats,10 rats were randomly selected as the blank control group,and the remaining rats were fed with high-fat and high-sugar feed for 4 weeks,intraperitoneally injected with STZ(11 mg/mL,5 mL/kg),and kept feeding for 4 weeks. For the successful modeling,42 rats were randomly divided into 4 groups:10 model control group,11 Ganlu Xiaoke Capsule low-dose group(0.93 g/kg crude drug),and Ganlu Xiaoke Capsule high-dose group (1.86 g/kg crude drug) 11 rats,metformin combined with valsartan control group (180 mg/kg,14.4 mg/kg) 10 rats. The intragastric volume of each administration group was 10 mL/kg,the blank control group and the model control group were intragastrically given equal volumes of distilled water. After 8 weeks of treatment,urine protein was measured for 24 hours. Blood was collected from the tail vein to measure random blood glucose. 10% chloral hydrate (3 mL/kg) anesthetize,take blood from the abdominal aorta,test blood urea nitrogen (BUN),blood creatinine (Scr),and finally take the kidney and fix it with formal collar for HE staining. Immunohistochemical staining was used to detect the expression of JAK2 and STAT3 in kidney tissue. [Results] This study showed that Ganlu Xiaoke Capsule can significantly reduce the expression of JAK2 and STAT3 in the kidney tissue of the diabetic nephropathy rat models,reduce the glomerular enlargement and basement membrane hyperplasia caused by diabetes,and reduce the content of urine protein,Scr and BUN in diabetic nephropathy rat models,and the above pharmacological effects are positively correlated with the effect of Ganlu Xiaoke Capsule in lowering blood sugar. [Conclusion] Ganlu Xiaoke Capsule can reduce the random blood glucose level of diabetic rats and regulate the expression of JAK2 and STAT3 in the kidney tissues,reduce the pathological damage of high blood sugar to kidney tissue,improve kidney function,and ultimately produce a protective effect on the kidneys of diabetic rats. |
|
|
|
|
|
|
|
