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| Effect of enema of Shaoyao Gancao Decoction in ketamine cystitis rats |
| Hits 1332 Download times 681 Received:May 13, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.11.18 |
| Key Words
Shaoyao Gancao Decoction;ketamine cystitis;oxidative stress;inflammatory factor |
| Author Name | Affiliation | E-mail | | ZHENG Dongxiang | Department of Urology, Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, China | | | ZHONG Liang | Department of Urology, Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, China | | | HUANG Zhifeng | Department of Urology, Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, China | | | ZENG Jianfeng | Department of Urology, Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, China | | | WU Song | Department of Urology, Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan 528400, China | jimzheya@163.com |
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| Abstract
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| [Objective] To investigate the effect and mechanism of Shaoyao Gancao Decoction enema on ketamine associated cystitis(KC) model rats. [Methods] Forty male SD rats were randomly divided into blank group,model group,levofloxacin group and Shaoyao Gancao Decoction group,with 10 rats in each group. KC model was established by intraperitoneal injection of ketamine for 12 weeks. After successful modeling,Shaoyao Gancao Decoction group was treated by Enema with 5 mL/kg,the positive control group was given levofloxacin,once a day for four weeks. Bladder urodynamic test was performed to compare the changes of residual urine volume,maximum bladder volume,maximum bladder pressure and bladder compliance in each group;pain threshold was detected and compared;He staining and ELISA were used to detect the pathological changes of bladder tissue and the levels of IL-1 β,IL-6 and TNF-α in serum. Western-blot was used to detect the protein expression of NF-κB p65,Nrf2 and COX-2 in bladder tissue. TransAMTM ELISA was used for the detection of DNA binding activity of NF-κB p65 and Nrf2. [Results] Compared with the model group,the maximum bladder volume,residual urine volume and bladder compliance in the Shaoyao Gancao Decoction group were significantly decreased,the maximum bladder internal pressure was increased,and the pain threshold was higher than model group (P<0.05). And the pathological damage of bladder tissue was improved,the expression of IL-1 β,TNF-α,IL-6 in serum and NF-κB p65,COX-2 protein was decreased,the expression of Nrf2 was up-regulated,while NF-κB p65 activity was decreased and Nrf2 activity was increased (P<0.05). [Conclusion] Shaoyao Gancao Decoction can inhibit oxidative stress and inflammatory reaction in KC model rats by regulating NF-KB/COX-2/Nrf2 signaling pathway,improve bladder function and reduce bladder tissue damage. |
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