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| Research of repair effect and mechanism of Anchang Yuyang decoction on intestinal mucosal barrier in ulcerative colitis rats |
| Hits 1223 Download times 682 Received:October 11, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.12.21 |
| Key Words
Anchang Yuyang decoction;ulcerative colitis;Occludin;Claudin-1;IL-13/JAK1/STAT6 signaling pathway |
| Author Name | Affiliation | E-mail | | SUN Dajuan | Department of Spleen and Gastric Diseases, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250000, China
The First Clinical College of Shandong University of Traditional Chinese Medicine, Jinan 250000, China | | | WANG Hanlu | The First Clinical College of Shandong University of Traditional Chinese Medicine, Jinan 250000, China | | | WANG Shuai | Department of Spleen and Gastric Diseases, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250000, China | | | CHI Lili | Department of Spleen and Gastric Diseases, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250000, China | chililiyl@163.com |
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| Abstract
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| [Objective] To observe the effect of Anchang Yuyang decoction on Occludin, Claudin-1 gene, protein and IL-13/JAK1/STAT6 signal pathway in ulcerative colitis rats, and to explore the mechanism of repairing intestinal mucosal barrier.[Methods] The 36 SD rats were randomly divided into 6 groups:blank group, model group, mesalazine group and Anchang Yuyang decoction low, medium and high dose group. After 4.5% DSS solution was used to establish the model and the rats were killed by gavage. The pathological conditions of colonic mucosa were examined by HE. The expressions of Occludin, Claudin-1 gene and protein were detected by RT-PCR and Western Blot, and the gene expressions of IL-13, JAK1, STAT6 were detected by RT-PCR.[Results] The score of histopathology was significantly decreased in all treatment groups (P<0.01). The colonic structure is improved and inflammatory cell infiltration was reduced, also Occludin, Claudin-1 gene and protein expression was raised in the treatment group (P<0.01). The gene expression of IL-13/JAK1/STAT6 pathway was activated to different degrees in other groups except the low-dose group(P<0.05 or P<0.01). The high-dose group and the mesalazine group had the most significant effects (P<0.05 or P<0.01). The correlation analysis showed that there was a negative correlation between the expression levels of Occludin, Claudin-1 and JAK1, STAT6(r=-0.664, r=-0.654, r=-0.629, r=-0.637, P<0.05), positive correlation with the expression levels of IL-13 (r=0.888, r=0.983, P<0.05).[Conclusion] Anchang Yuyang decoction can significantly promote intestinal mucosal repair in UC rats, the mechanism of which may be related to the regulation of the IL-13/JAK1/STAT6 signaling pathway and the up-regulation of the expression levels of Occludin and Claudin-1 in colon tissues. |
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