|
| Effects of Xianqi Xuanning Granule on the expression of aquaporin-2 in the cochlea of guinea pig with endolymphatic hydrops |
| Hits 653 Download times 410 Received:June 28, 2021 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2021.12.22 |
| Key Words
Xianqi Xuanning Granule;endolymphatic hydrops;aquaporin-2 |
| Author Name | Affiliation | E-mail | | WU Xuerun | Department of Practical Teaching, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YANG Dong | Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300050, China | | | WANG Mingxin | Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300050, China | | | ZHAO Shujun | Key Laboratory of Hormones and Development of Ministry of Health, Institute of Endocrinology of ZHU Xianyi Memorial Hospital of Tianjin Medical University, Tianjin 300134, China | | | YE Yan | Key Laboratory of Hormones and Development of Ministry of Health, Institute of Endocrinology of ZHU Xianyi Memorial Hospital of Tianjin Medical University, Tianjin 300134, China | 616064799@qq.com |
|
| Abstract
|
| [Objective] To study the influence of Xianqi Xuanning Granule on the morphology of cochlea and the expression of aquaporin-2 (AQP2) in guinea pigs with endolymphatic hydrops, and to discuss the mechanism of treating meniere's disease.[Methods] Fifty guinea pigs were randomly divided into the control group, model group, low dose treatment group(LT), moderate dose treatment group (MT), high dose treatment group (HT) on average. All guinea pigs except those in the control group were used for making endolymphatic hydrops models with intraperitoneal injection of desmopressin acetate. Three treatment groups were treated with different dosage of Xianqi Xuanning Granule by feeding way, separately 6.1, 12.2, 24.4 g/kg, while those in the control and model groups were treated with physiological saline. Auditory function was tested by auditory brain response (ABR). The endolymphatic hydrops in cochlea were evaluated by hematoxylin-eosin (HE) staining, and the ratio of the scala media area to the total area of that and scala vestibuli was calculated. The expression of AQP2 protein in guinea pig cochlea was detected by immunohistochemistry and Western Blot.[Results] The ABR threshold in the model group increased because of hearing impairment, which of each treatment group was similar to that of the control group (P>0.05). R value in the model group was higher than that in the control group (P<0.01). There was no statistical difference between the treatment groups and the control group (P>0.05). The damage of cochlea hair cells occurred in model group by scanning electron microscope, and they appeared to be normal in the treatment groups. The AQP2 expression of the model group was higher than those of the control group and three treatment groups by immunohistochemistry (P<0.01). Western Blot results showed that the expression of AQP2 in the model group and LT group was higher than that in the control group, while it in HT group was significantly lower than that in the control group (P<0.01).[Conclusion] Xianqi Xuanning Granule could suppress the development of endolymphatic hydrops in guinea pigs, and it might be associated with the down-regulation of the AQP2 expression. |
|
|
|
|
|
|
|
