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| Effect of Jiangtang Xiaoke Granule on liver glycogen content and hepatic glucose metabolism-related genes in type 2 diabetic mice |
| Hits 701 Download times 563 Received:September 28, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.01.22 |
| Key Words
Jiangtang Xiaoke Granule;type 2 diabetes mellitus;hepatic glycometabolism;insulin receptor substrate-2;liver glycogen |
| Author Name | Affiliation | E-mail | | ZHANG Yi | Beijing Open University, Beijing 100081, China | | | ZHAO Dandan | Beijing University of Chinese Medicine, Beijing 100029, China | | | MO Fangfang | Beijing University of Chinese Medicine, Beijing 100029, China | | | GAO Sihua | Beijing University of Chinese Medicine, Beijing 100029, China | gaosihua1216@163.com |
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| Abstract
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| [Objective] To investigate the effect of Jiangtang Xiaoke Granule （JTXKG） on liver glycogen content andhepatic insulin receptor substrate-2 （IRS-2） mRNA，protein kinase B，PKB/Akt （Akt） mRNA，glycogen synthase kinase 3α （GSK-3α） mRNA expression in type 2 diabetic KK-Ay mice. [Methods] KK-Ay mice，male，8 weeks old，were fed by high-fat-diet for 4 weeks，T2DM model were established by FBG≥13.9 mmol/L. The T2DM mice were then randomly divided into model group，pioglitazone group and JTXKG groups in low （1.75 g/kg），medium （3.50 g/kg），and high （7.00 g/kg） doses. The normal group had C57BL/6J in it and feed by normal diet. After 10-week treatment，the average daily food intake and the rate of weight gain were weighed and calculated. The liver glycogen was examined by Periodic Acid-Schiff stain. The expressions of GSK-3α was examined by real-time fluorescence quantitative PCR，and the effects of JTXKG on the above indexes were observed. [Results] Compared with model group，T2DM mice supplemented with 3.50 g/kg dose of JTXKG can lower GSK-3α mRNA expression （P<0.01） and raise IRS-2 mRNA （P<0.01），Akt mRNA （P<0.01） expression to restore more liver glycogen （P<0.05）. The 7 g/kg dose of JTXKG also can raise IRS-2 mRNA and lower hepatic GSK-3α mRNA expression of T2DM mice significantly （P<0.01）. [Conclusion] JTXKG can raised the liver glycogen synthetize and repertory function in T2DM mice by regulating hepatic glycometabolism related gene expressions. |
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