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| Guizhi plus Gegen Decoction improves rat intervertebral disc endplate chondrocyte degeneration through SDF-1/CXCR4 signal axis |
| Hits 728 Download times 480 Received:October 10, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.01.23 |
| Key Words
intervertebral disc;Guizhi plus Gegen Decoction;endplate chondrocytes;cell degeneration;SDF-1;CXCR4 |
| Author Name | Affiliation | E-mail | | JIANG Wei | Department of Traumatology and Orthopedics, The Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830002, China | | | WANG Jun | Department of Traditional Chinese Medicine, The Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830002, China | | | REN Zheng | Department of Traumatology and Orthopedics, The Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830002, China | 495595832@qq.com |
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| Abstract
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| [Objective] To investigate the effect and mechanism of Guizhi plus Gegen Decoction in the degeneration of rat endplate chondrocytes induced by mechanical stimulation in vitro. [Methods] Isolate and culture rat endplate chondrocytes，the Flexcell FX-5000TM cell stress loading system was used to establish an endplate chondrocyte degeneration model，the experiment was divided into control group，model group，Guizhi plus Gegen Decoction group （200 mL/L Guizhi plus Gegen Decoction-containing serum treated cells for 24 h before modeling），Guizhi plus Gegen Decoction+SDF-1α group （200 mL/L Guizhi plus Gegen Decoction and 100 μg/L SDF-1α treated cells for 24 h before modeling）. CCK-8 method and flow cytometry detect cell proliferation and apoptosis，toluidine blue staining and phalloidin staining to observe cell morphology changes，immunofluorescence staining was used to detect the positive expression of CXC chemokine receptor 4 （CXCR4） in the cells. qRT-PCR and Western blot were used to detect the changes in the expression levels of type II collagen fibers α1 （COL2A1） and SOX9 mRNA and protein in the cells. [Results] Compared with the control group，the cell activity of rat endplate chondrocytes decreased after mechanical stimulation （P<0.05），and the apoptosis rate increased （P<0.05），and the cells degenerated，and the shape changed from polygon to spindle，and the relative expression of COL2A1 and SOX9 mRNA and protein in the cells decreased （P<0.05）；compared with the model group，the activity of endplate chondrocytes treated with Guizhi plus Gegen Decoction increased （P<0.05），the apoptosis rate was decreased （P<0.05），the cell regression was inhibited，and the cell morphology changes were alleviated. At the same time，the relative expression of COL2A1 and SOX9 mRNA and protein increased （P<0.05），but no improvement was found in the cells co-cultured with Guizhi plus Gegen Decoction and SDF-1α. [Conclusion] Guizhi plus Gegen Decoction can intervene in the SDF-1/CXCR4 signaling pathway in vitro，block the binding of SDF-1 to the CXCR4 receptor on the surface of chondrocytes，and reduce the degeneration of chondrocytes in the endplate of rat intervertebral disc. |
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