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| Study on the anti-osteoporosis effect of ligustrum lucidum polysaccharide in ovariectomized mice |
| Hits 679 Download times 425 Received:September 26, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.01.24 |
| Key Words
ligustrum lucidum polysaccharide;osteoporosis;osteoclast |
| Author Name | Affiliation | E-mail | | WEI Qiu | Key Laboratory of Chinese Pharmacology of the Ministry of Education, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HOU Jiahua | Key Laboratory of Chinese Pharmacology of the Ministry of Education, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | QIN Xiaoyan | Key Laboratory of Chinese Pharmacology of the Ministry of Education, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YANG Yun | Key Laboratory of Chinese Pharmacology of the Ministry of Education, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | AN Ran | Key Laboratory of Chinese Pharmacology of the Ministry of Education, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MAO Haoping | Key Laboratory of Chinese Pharmacology of the Ministry of Education, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | haoping_mao@126.com |
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| Abstract
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| [Objective] To study the effect of ligustrum lucidum polysaccharide on osteoporosis in mouse caused by ovariectomized. [Methods] Ligustrum lucidum polysaccharides （3 g/kg and 6 g/kg，calculated as crude drugs） were given to ovariectomized mice for 8 weeks. The bone mineral density and bone microstructure of femurs were measured by DXA and microCT. Calcium and phosphorusinplasma were measured by automatic biochemical instrument. Bone marrow-derived mononuclear cells were cultured in vitro，and 25 μg/L MCSF and 50 μg/L RANKL were used to induce osteoclast differentiation. At the same time，ligustrum lucidum polysaccharides （1×10-3，1×10-4，1×10-5 mg/mL） were used for intervention treatmentto study the effect of ligustrum lucidum polysaccharide on the differentiation of mononuclear cells into osteoclasts. [Results] After 8 weeks of administration，compared with sham group，the plasma levels of calcium and phosphorus in the model group were significantly increased （P<0.05）. Compared with the model group，plasma calcium and phosphorus levels inestradiol group，3 g/kg and/or 6 g/kg ligustrum lucidum polysaccharide group were significantly decreased （P<0.05）. The results of dual-energy rays showed that the bone mineral density of the femur in the estrogen group，3 g/kg and 6 g/kg ligustrum lucidum polysaccharide groups were significantly increased than that of the model group. The results of micro CT showed that the bone microstructure of the femoral metaphysis of mice in the 3 g/kg and 6 g/kg groups of ligustrum lucidum polysaccharide was significantly improved. In vitro，compared with the model group，the TRAP enzyme activity in the culture supernatant of the 1×10-3，1×10-4，and 1×10-5 mg/mL ligustrum lucidum polysaccharide groupswas significantly reduced （P<0.05），but there was no significant dose-dependent resistant relationship. [Conclusion] Ligustrum lucidum polysaccharide may play an anti-osteoporotic effect by inhibiting osteoclast differentiation and regulating calcium and phosphorus metabolism. |
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