|
| Effects of anemarrhena total saponins on sex hormone levels in female SD rats with precocious puberty and its effects on HPGA axis |
| Hits 693 Download times 407 Received:September 29, 2021 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2022.01.25 |
| Key Words
anemarrhena total saponins;female;precocious puberty;sex hormone;hypothalamic-pituitary-gonadal axis |
| Author Name | Affiliation | | GAO Jing | Department of Endocrinology, Genetics and Metabolism, Zhengzhou Children's Hospital/Henan Children's Hospital, Zhengzhou 450000, China | | WANG Yanhong | Department of Endocrinology, Genetics and Metabolism, Zhengzhou Children's Hospital/Henan Children's Hospital, Zhengzhou 450000, China | | XU Bangli | Department of Neonatology, Zhengzhou Children's Hospital/Henan Children's Hospital, Zhengzhou 450000, China |
|
| Abstract
|
| [Objective] To observe the effect of anemarrhena total saponins on the level of sex hormones in female SD rats with precocious puberty and the effect on the hypothalamus-pituitary-gonad axis （HPGA）. [Methods] A female precocious SD rat model was constructed and randomly divided into a model group，an anemarrhena total saponins low-dose group （Zhi-low group），an anemarrhena total saponins high-dose group （Zhi-high group），a positive control group，and a normal group. After corresponding intervention，record the opening time of the vulva and the time of the first estrus in each group of rats. Take the sex organs of the rats to calculate the uterus and ovarian indexes. HE staining to observe the morphological changes of the rat ovary. ELISA to detect serum levels of sex hormones follicle-forming hormone （FSH），luteinizing hormone （LH） and estradiol （E2） in rats. QPCR and Western blot detection of rat hypothalamic tumor metastasis suppressor genes kissin-1 （KISS-1），G protein-coupled receptor 54 （GPR54），gonadotropin releasing hormone （GnRH） and its receptor （GnRHR） mRNA and protein expression. [Results] Compared with the normal group，the vulva opening time and the first estrus interval time of the model group were shorter，the uterus and ovary indexes were higher，the number of corpus luteum was higher，the serum FSH，LH，and E2 levels were higher，and the upper thalamus KISS-1，GPR54，GnRH，GnRHR mRNA and protein expression levels were higher （P<0.05），compared with the model group，the vulva opening time and the first estrus interval time were longer in the Zhi-low group，Zhi-high group，and positive control group. Uterus and ovary index is low，the number of corpus luteum is low，serum FSH，LH，E2 levels are low，and the expression of KISS-1，GPR54，GnRH，GnRHR mRNA and protein in the epithalamus is low （P<0.05），in comparison，the opening time of the vulva in the Zhi-high group and the positive control group was longer than the first estrus interval，the uterus and ovarian indexes were lower，the number of corpus luteum was lower，the serum FSH，LH，and E2 levels were lower，and the hypothalamus KISS-1，GPR54，GnRH，GnRHR mRNA and protein expression are lower （P<0.05），compared with Zhi-high group，the time of opening the vulva and the first estrus interval is longer in the positive control group，and the index of uterus and ovary is lower，the number of corpus luteum is small，the level of serum FSH，LH，E2 is low，and the expression of KISS-1，GPR54，GnRH，GnRHR mRNA and protein in the upper thalamus is low （P<0.05）. [Conclusion] The anemarrhena total saponins can regulate the level of sex hormones in female precocious SD rats，and have an inhibitory effect on the HPGA axis. |
|
