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| Study on the quality of mulberry leaves based on UPLC-DAD fingerprint combined with chemometrics and determination of 6 index components |
| Hits 611 Download times 464 Received:October 28, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.02.24 |
| Key Words
mulberry leaf;UPLC-DAD;fingerprint atlas;similarity evaluation;chemometrics;determination of content |
| Author Name | Affiliation | E-mail | | YU Pengxin | Pharmaceutical Engineering College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHEN Ying | Pharmaceutical Engineering College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Lijuan | Pharmaceutical Engineering College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Heshui | Pharmaceutical Engineering College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China
Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering, Tianjin 301617, China | hs_yu08@163.com | | SONG Xinbo | Pharmaceutical Engineering College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China
Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering, Tianjin 301617, China | | | LI Zheng | Pharmaceutical Engineering College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China
Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering, Tianjin 301617, China | |
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| Abstract
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| [Objective] A UPLC-DAD fingerprint method was established to comprehensively evaluate the quality of mulberry leaf combined with chemometrics and multi-index content determination.[Methods] The chromatographic column was Agilent EC-C18 column (2.1 mm×100 mm, 1.7 μm), and the column temperature was set at 35℃. The detection wavelength were set at 290 nm (for methyl gallate and cryptochlorogenic acid) and 360 nm (for rutin, isoquercitrin, kaempferol-3-O-rutinoside, astragalin). The mobile phase was composed of acetonitrile-0.2% phosphate acid solution in gradient elution manner at a flow rate of 0.2 mL/min and Sample volume 2 μL. Establish UPLC-DAD fingerprints of 22 batches of mulberry leaf medicinal materials, using cluster analysis and principal component analysis and other chemometric methods, as well as methyl gallate, cryptochlorogenic acid, rutin, isoquercitrin, kaempferol-3-O-rutinoside and astragalus glycosides were used to evaluate the quality of mulberry leaves.[Results] Twenty-two batches of mulberry leaves UPLC-DAD fingerprints confirmed 16 common peaks, the similarities of mulberry leaf medicinal materials from different batches and the control fingerprints are quite different. And 6 shared peaks were identified by the reference substance, the results showed that the separation of the 6 index components was good, and each component had a wide linear range and a good linear relationship (r2>0.999), with good precision, stability, repeatability and sample recovery.[Conclusion] Using UPLC-DAD technology to establish a fingerprint spectrum combined with 6 index component content determination method is convenient, accurate and reproducible, which could be used for quality evaluation of mulberry leaves. |
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