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| Study on the mechanism of Xiaoyan Decoction inhibiting proliferation and promoting apoptosis of lung adenocarcinoma A549 cells through WNT pathway |
| Hits 521 Download times 405 Received:November 25, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.03.20 |
| Key Words
Xiaoyan Decoction;WNT signaling pathway;lung adenocarcinoma;proliferation and apoptosis |
| Author Name | Affiliation | E-mail | | ZHENG Xu | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinses Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | HAN Yanyan | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinses Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | GU Lili | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinses Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | LI Chong | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinses Medicine Acupuncture and Moxibustion, Tianjin 300381, China | lc740922@163.com |
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| Abstract
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| [Objective] To investigate the possible mechanism of "Xiaoyan Decoction" classical medicine of strengthening the body resistance,removing blood stasis and detoxifying method,to inhibit A549 cell proliferation and promote cell apoptosis in human lung adenocarcinoma. [Methods] A549 cells were cultured in vitro,and the cytotoxicity,survival rate and apoptosis rate of the control group,Xiaoyan Decoction group,Xiaoyan Decoction plus WNT pathway agonist group,XAV939 group and WNT pathway agonist group were detected by LDH,CCK8 and flow cytometry. The expression levels of β-catenin,c-myc,Bcl-2 and Caspase-3 related genes in WNT pathway were determined by PCR. WNT pathway activity was detected by luciferase reporter gene assay in control group,Xiaoyan Decoction group and WNT pathway inhibitor XAV939 group. [Results] Xiaoyan Decoction can inhibit the proliferation of A549 cells and promote apoptosis,while Xiaoyan Decoction plus WNT pathway agonist group can weaken the above effect,meanwhile,Xiaoyan Decoction can significantly reduce the expression levels of β-catenin and c-myc in A549 cells in a dose-dependent manner,inhibit the activity of WNT pathway,and promote the apoptosis of tumor cells by reducing the expression of Bcl-2 and enhancing the expression of caspase-3. [Conclusion] Xiaoyan Decoction may inhibit the proliferation and promote apoptosis of A549 cells by inhibiting the WNT signaling pathway. |
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