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| Study on the effect of cordycepin on fracture healing by inhibiting oxidative stress and inflammation |
| Hits 692 Download times 373 Received:December 27, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.03.22 |
| Key Words
cordycepin;fracture;oxidative stress;inflammation |
| Author Name | Affiliation | E-mail | | LIU Lizhu | Department of Trauma Surgery, Second Affiliated Hospital of Hainan Medical College, HaiKou 570311, China | | | LI Chaoyi | Department of Trauma Surgery, Second Affiliated Hospital of Hainan Medical College, HaiKou 570311, China | li35499015@126.com | | LIN Shiwei | Department of Trauma Surgery, Second Affiliated Hospital of Hainan Medical College, HaiKou 570311, China | | | QU Ye | Department of Trauma Surgery, Second Affiliated Hospital of Hainan Medical College, HaiKou 570311, China | | | XU Haijun | School of Basic Medicine and Life Sciences, Hainan Medical University, HaiKou 571199, China | |
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| Abstract
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| [Objective] To explore the effect and mechanism of cordycepin in the treatment of fracture healing. [Methods] 50 SPF SD rats were selected to established fracture healing model,which were treated with cordycepin. The femurs of fracture rats were examined by Micro-CT and quantitatively analyzed by Tb.Th,BV/TV,BMDand Tb.N,the contents of bone alkaline phosphatase(BAP),RANKL and tartrate-resistant acid phosphatase(TRAP) in tissues were detected by commercial kit. The contents of bone alkaline phosphatase(ALP),bone alkaline phosphatase(RANKL) and tartrate-resistant acid phosphatase(TRAP) in tissues were detected by commercial kit. The pathological changes of femur of fract'ure rats were detected by hematoxylin-eosin staining,safranin O staining and Masson staining. The contents of interleukin-1β,interleukin-6 and tumor necrosis factor-α in serum of fracture rats were detected by ELISA kit. The contents of MDA in serum and MDA and CAT in tissue of fracture rats were detected by commercial kit. [Results] Compared with the model group,the fracture site of rats treated with cordycepin healed obviously,and Tb.Th,BV/TV,BMD and Tb.N increased significantly(P<0.05),and cordycepin significantly decreased the contents of bone alkaline phosphatase (BAP),RANKL and tartrate resistant acid phosphatase (TRAP) in femoral tissue of fracture rats in a dose-dependent manner. The HE staining results showed that the cordycepin 5mg/kg and 10mg/kg group rats observed lamellar bones arranged neatly and the woven bones were reduced;the safranine fast green staining results showed that the cordycepin 5 mg/kg and 10 mg/kg groups had cartilage. The tissue decreased and the osteogenic tissue increased;Masson staining results showed that mature bone tissue was observed in cordycepin 5 mg/kg and 10 mg/kg groups. Cordycepin significantly decreased the contents of IL-1β,IL-6 and TNF-α in fracture rats in a dose-dependent manner,and significantly decreased the contents of MDA in serum and MDA and CAT in tissues of fracture rats in a dose-dependent manner. [Conclusion] Cordycepin has a potential promoting effect on fracture healing,and this effect may be related to antioxidant stress and inflammation. |
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