|
| Regulatory effect of matrine on SCAP/SREBP1 signal pathway in mice with liver metastasis of colon cancer |
| Hits 601 Download times 461 Received:December 22, 2021 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2022.03.23 |
| Key Words
matrine;liver metastasis of colon cancer;SCAP/SREBP1 signal pathway |
| Author Name | Affiliation | E-mail | | MA Yi | Department of Oncology, The First Hospital of Hunan University of Chinese Medicine, Changsha 410000, China | | | XIE Qiong | Department of Oncology, The First Hospital of Hunan University of Chinese Medicine, Changsha 410000, China | 707015967@qq.com |
|
| Abstract
|
| [Objective] To study the inhibitory effect of matrine on liver metastasis of colon cancer and its regulation on SCAP/SREBP1 signal pathway in mice. [Methods] The 72 mice were randomly divided into blank control group,liver metastasis model group,low,middle and high dose matrine groups and capecitabine group,with 12 mice in each group. The mouse model of colon cancer liver metastasis was established by intrasplenic injection of HCT116 cells. The low,middle and high dose matrine groups were given 12.5,25,50 mg/kg,for 21 days. Capecitabine group was given capecitabine by intragastric administration according to 267 mg/kg. The liver weight and the number of liver metastatic nodules were measured. The levels of transforming growth factor(TGF-β1) and interleukin (IL)-6 in liver tissue were measured by enzyme-linked immunosorbent assay (ELISA). Liver histopathology was examined by hematoxylin-eosin (HE) staining. The levels of SCAP and SREBP1 mRNA in tumor tissue were determined by real-time quantitative polymerase chain reaction (PCR). The levels of SCAP and SREBP1 in tumor tissue were determined by Western blot. [Results] Compared with the blank control group,the liver weight,the number of hepatic metastatic nodules,the levels of TGF-β1 and IL-6 in liver tissue,and the levels of SCAP,SREBP1 mRNA and protein in tumor tissue in liver metastasis model group were significantly higher than those in control group(P<0.05). Compared with the liver metastasis model group,the liver weight,the number of liver metastatic nodules,the levels of TGF-β1 and IL-6 in liver tissue,and the levels of SCAP,SREBP1 mRNA and protein in tumor tissue in each dose group were significantly lower than those in the liver metastasis model group(P<0.05). [Conclusion] Matrine can significantly inhibit liver metastasis of colon cancer and alleviate liver histopathological changes,and its mechanism may be related to the regulation of SCAP/SREBP1 signal pathway. |
|
|
|
|
|
|
|
