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| Enzymatic hydrolysis of hirudo and pheretima residue and evaluation of anticoagulant activity of the product |
| Hits 530 Download times 376 Received:December 23, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.03.24 |
| Key Words
hirudo;pheretima;enzymolysis;fibrinogen plate lysis;thrombin titration |
| Author Name | Affiliation | E-mail | | LI Shunan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JIN Hui | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | NI Kailing | Mudanjiang Youbo Pharmaceutical Co., Ltd., Mudanjiang 157000, China | | | YU Yang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Zheng | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Na | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | nwang201098@163.com |
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| Abstract
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| [Objective] The technological conditions of enzymatic hydrolysis of hirudo and pheretima residue by protease were investigated,and the anticoagulant activity of the hydrolysate was evaluated.[Methods] Alkaline protease,acid protease and neutral protease were used to hydrolyze the mixed residues. Thrombin titration and fibrinogen plate lysis were used to evaluate the anticoagulant activity of the hydrolysates in vitro to determine the optimal enzyme type. The single factor experiment was carried out to optimize the enzymolysis process by selecting pH,enzyme substrate ratio,enzymolysis time and enzymolysis temperature as factors. [Results] Compared with the drug residue control group and protease control group,the mixture of hirudo and pheretima showed strong anticoagulant activity. Among them,alkaline protease hydrolysate had the best anticoagulant effect. The optimum conditions were as follows:enzyme substrate ratio 0.95%,pH 11.4,enzymolysis temperature 45℃,enzymolysis time 4 h. [Conclusion] The anticoagulant activity of the hydrolysate of hirudo and pheretima residue was the strongest after alkaline protease hydrolysis. This experiment provides experimental basis and development direction for the reuse of waste drug residue. |
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