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| Regulatory effect of Bushen Huoxue Decoction on Nrf2/Keap1 pathway in rats with endometriosis |
| Hits 582 Download times 342 Received:December 30, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2022.04.20 |
| Key Words
Bushen Huoxue Decoction;endometriosis;Nrf2/Keap1 pathway |
| Author Name | Affiliation | | LI Zhenhua | Department of Traditional Chinese Medicine and Gynecology, The First People's Hospital of Chenzhou, Chenzhou 423000, China | | LI Jiao | Department of Traditional Chinese Medicine and Gynecology, The First Hospital of Hunan University of Chinese Medicine, Changsha 430000, China | | ZHANG Jian | Department of Traditional Chinese Medicine and Gynecology, The First People's Hospital of Chenzhou, Chenzhou 423000, China |
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| Abstract
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| [Objective] To study the regulatory effect of Bushen Huoxue Decoction on Nrf2/Keap1 pathway in rats with endometriosis.[Methods] The 90 rats were randomly divided into sham operation group,model group,low,middle and high dose Bushen Huoxue Decoction groups and danazol group. Endometriosis model rats were established by autologous endometrial transplantation. Bushen Huoxue Decoction low,middle and high dose groups were given Bushen Huoxue Decoction according to 2.5,5 and 10 g/kg,and danazol group was given danazol (0.02 g/kg) once a day. After continuous intervention for 4 weeks,the levels of estradiol (E2) and cancer antigen (CA) 125 in serum and the levels of interleukin-6 (IL-6),interleukin-8 (IL-8) and prostaglandin F2α (PGF-2α) in peritoneal lavage fluid were detected by enzyme-linked immunosorbent assay (ELISA). The histopathological changes of uterus were detected by hematoxylin-eosin (HE) staining. The levels of Nrf2 and Keap1 mRNA in ectopic uterine tissue of rats with endometriosis were detected by real-time polymerase chain reaction (PCR). The levels of Nrf2 and Keap1 in ectopic uterine tissue of rats were measured by Westrn blot.[Results] Compared with the sham operation group,in model group,the levels of serum E2 and CA125 and the levels of IL-6,IL-8 and PGF-2 α in peritoneal lavage fluid were increased (P<0.05),Nrf2 mRNA and protein in ectopic uterus were increased (P<0.05),Keap1 mRNA and protein were decreased(P<0.05). Compared with the model group,the levels of E2 and CA125 in serum and the levels of IL-6,IL-8 and PGF-2 α in peritoneal lavage fluid,the levels of Nrf2 mRNA and protein in ectopic uterus were decreased(P<0.05),the levels of Keap1 mRNA and protein were increased(P<0.05),and the pathological symptoms such as thickening of ectopic endometrium alleviated in Bushen Huoxue Decoction groups.[Conclusion] Bushen Huoxue Decoction can reduce the levels of serum estradiol and CA125 and relieve abdominal inflammation in rats with endometriosis. The mechanism may be related to the regulation of Nrf2/Keap1 pathway. |
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